山东省泰山学者特聘教授

符军

教授
博士生导师
0532-67720928
个人简介 科研项目 学术论文 其他介绍

教育背景

博士:德累斯顿工业大学 2004–2012毕业院校:

硕士:德累斯顿工业大学 1998–2001毕业院校:

学士:西北农林科技大学 1987–1991

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工作经历

起止时间 单位名称

2014- 山东大学微生物技术国家重点实验室

2004–2014 德累斯顿工业大学生物技术中心

2002–2004 基因桥公司(Gene Bridges GmbH)

1991–1998 西北农林科技大学

科研项目

应用自身噬菌体重组蛋白建立枯草芽孢杆菌DNA同源重组系统(国家自然科学基金面上项目, 31570094)

磁小体基因簇的克隆及应用于肿瘤靶向治疗(国家国际科技合作专项, 2015DFE32850)

杀线虫根际靶向生防工程菌的人工构建 (山东省自然科学基金重大基础研究项目,ZR2018ZC2261)

放线菌底盘适配性机理与产物高产机制研究 - 高效基因编辑与异源表达技术开发 (国家重点研发计划子课题 2018YFA0900403)

山东省泰山学者特聘专家计划 (2018-2022)

代表性论文

1. Jia Yin, Wentao Zheng, Yunsheng Gao, Chanjuan Jiang, Hongbo Shi, Xiaotong Diao, Shanshan Li, Hanna Chen, Hailong Wang, Ruijuan Li, Aiying Li, Liqiu Xia, Yulong Yin, A. Francis Stewart, Youming Zhang and Jun Fu.(2019)“Single-Stranded DNA-Binding Protein and Exogenous RecBCD Inhibitors Enhance Phage-Derived Homologous Recombination in Pseudomonas”iScience. 14:1-14.

2. Chaoyi Song, Ji Luan, Qingwen Cui, Qiuyue Duan, Zhen Li, Yunsheng Gao, Ruijuan Li, Aiying Li, Yue-Mao Shen, Yue-Zhong Li, Adrian Francis Stewart, Youming Zhang, Jun Fu, and Hailong Wang (2019)“Enhanced heterologous spinosad production from a 79-kb synthetic multi-operon assembly”ACS Synthetic Biology, 8 (1):137-147

3. Gulce Itir Percin, Jiri Eitler, Andrea Kranz, Jun Fu, Jeffrey W. Pollard, Ronald Naumann & Claudia Waskow (2018) “CSF1R regulates the dendritic cell pool size in adult mice via embryo-derived tissue-resident macrophages”Nature Communications. 9(1):5279

4. Yuhan Zhang, Ruxin Liu, Kaiyue Tian, Zeng Wang, XiaYang, Dongsheng Gao, Youming Zhang, Jun Fu*, Hailong Wang*, Jun Zhao* (2018)“Fiber2 and hexon genes are closely associated with the virulence of the emerging and highly pathogenic fowl adenovirus 4”Emerging Microbes & Infections. 7(1):199. doi: 10.1038/s41426-018-0203-1.

5. Hailong Wang, Zhen Li,Ruonan Jia,Jia Yin, Aiying Li,Liqiu Xia,Yulong Yin, Rolf Müller, Jun Fu, A. Francis Stewart and Youming Zhang (2018) “ExoCET: Exonuclease in vitro assembly combined with RecET recombination for highly efficient direct DNA cloningfrom complex genomes”Nucleic Acids Research. 2018 Mar 16; 46(5):e28.

6. Oliver Baker, Sarah Tsurkan, Jun Fu, Barbara Klink, Andreas Rump, Mandy Obst, Andrea Kranz, Evelin Schr?ck, Konstantinos Anastassiadis and A. Francis Stewart (2017) “The contribution of homology arms to nuclease-assisted genome engineering.”Nucleic Acids Research 2017 Jun 5. doi: 10.1093/nar/gkx497

7. Wenli Zhang, Jun Fu, Jing Liu, Hailong Wang, Maren Schiwon, Sebastian Janz, Lukas Schaffarczyk, Lukas von der Goltz, Eric Ehrke-Schulz, Johannes D?rner, Manish Solanki, Philip Boehme, Thorsten Bergmann, Andre Lieber, Chris Lauber, Andreas Dahl, Andreas Petzold, Youming Zhang, A. Francis Stewart and Anja Ehrhardt (2017) “An Engineered Virus Library as a Resource for the Spectrum-wide Exploration of Virus and Vector Diversity”Cell Reports. 19(8):1698-1709.

8. Hailong Wang, Zhen Li, Ruonan Jia, Yu Hou, Jia Yin, Xiaoying Bian, Aiying Li, Rolf Müller, A Francis Stewart, Jun Fu & Youming Zhang (2016) "A concerted pipeline for direct cloning of biosynthetic gene clusters and engineering for heterologous expression"Nature Protocols 11(7): 1175-90.

9. Oliver Baker, Ashish Gupta, Mandy Obst, Youming Zhang, Konstantinos Anastassiadis, Jun Fu and A. Francis Stewart (2016) “RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses” Scientific Reports 2016 May 24;6:25529. doi: 10.1038/srep25529.

10. Yin, J., Zhu, H., Xia, L., Ding, X., Hoffmann, T., Hoffmann, M., Bian, X., Müller, R., Fu, J., Stewart, A.F., Zhang, Y. (2015) “A new recombineering system for Photorhabdus and Xenorhabdus”Nucleic Acids Research2015 Mar 31; 43(6):e36.

11. Wang, H., Bian, X., Xia, L., Ding, X., Müller, R., Zhang, Y., Fu, J. and Stewart, A. F. (2014) “Improved seamless mutagenesis by recombineering using ccdB for counterselection"Nucleic Acids Research 2013 Dec 24.

12. Filipczyk, A., Gkatzis, K., Fu, J., Lickert, H., Anastassiadis, K. and Schroeder, T. (2013) " Biallelic Expression of Nanog Protein in Mouse Embryonic Stem Cells"Cell Stem Cell 13:12-13

13. Rostovskaya, M., Fu, J., Obst, M., Baer, I., Weidlich, S., Wang, H., Smith, A., Anastassiadis, K. and Stewart, A. F. (2012) “Transposon-Mediated BAC Transgenesis in Human ES Cells”Nucleic Acids Research, 2012 Jun 30. doi: 10.1093/nar/gks643

14. Fu, J., Bian, X., Hu, S., Wang, H., Huang, F., Seibert, P., Plaza, A., Xia, L., Müller, R., Stewart, A. F. and Zhang, Y. (2012) "Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting.”Nature Biotechnology 30: 440-446.

15. Bird, A. W., Erler, A., Fu, J., Maresca, M., Heriche, J. K., Zhang, Y., Hyman, A. A. and Stewart, A. F. (2011) "High-efficiency counterselection recombineering for site-directed mutagenesis in bacterial artificial chromosomes."Nature Methods 9:103-109.

16. Nedelkova, M., Maresca, M., Fu, J., Rostovskaya, M., Thiede, C., Anastassiadis, K., Sarov, M. and Stewart, A. F. (2011). "Targeted isolation of genomic regions by recombineering."Nucleic Acids Research 39:e137.

17. Daniel, K., Lange, J., Hached, K., Fu, J., Anastassiadis, K., Roig, I., Cooke, H. J., Stewart, A. F., Wassmann, K., Jasin, M., Keeney, S. and Toth, A. (2011). "Meiotic homologue alignment and its quality surveillance are controlled by mouse HORMAD1."Nature Cell Biology 13:599-610.

18. Skarnes, W. C., Rosen, B., West, A. P., Koutsourakis, M., Bushell, W., Iyer, V., Cox, R., Jackson, D., Severin, J., Biggs, P., Thomas, M., Mujica, A., Harrow, J., Fu, J., Nefedov, M., de Jong, P., Stewart, A. F. and Bradley, A. (2011). "A conditional knockout resource for genome-wide analysis of mouse gene function."Nature 474: 337-42.

19. Fu, J., Teucher, M., Anastassiadis, K., Skarnes, W. and Stewart, A. F. (2010)."A Recombineering pipeline to make conditional targeting constructs."Methods in Enzymology 477C: 125-144.

20. Fu, J., Wenzel, S. C., Perlova, O., Wang, J., Gross, F., Tang, Z., Yin, Y., Stewart, A. F., Müller, R. and Zhang, Y. (2008)."Efficient transfer of two large secondary metabolite pathway gene clusters into heterologous hosts by transposition." Nucleic Acids Research 36: e113.

所获专利

张友明、王海龙、符军、A. Francis Stewart(08.2017)基因组大片段直接克隆和DNA多分子组装新技术,国际发明专利申请号: PCT/CN2017/000483

STEWART, Adrian, Francis; FU, Jun; EHRHARDT, Anja; EHRKE-SCHULZ, Eric; ZHANG, Wenli “Adenoviral vectors” PCT/EP2017/058306

TU, Qiang; M?LLER, Rolf; STEWART, Francis; FU, Jun; ZHANG, Youming.“High efficiency of electrocompetent cells prepared at room temperature” PCT/EP2013/071546

FU, Jun; ZHANG, Youming; STEWART, Francis.“Direct cloning of DNA fragment from genomic DNA without library construction and screening” PCT/IB2011/052549

ZHANG, Youming; FU, Jun; BIAN, Xiaoying; STEWART, Francis; ZIPS, Daniel; M?LLER, Rolf “Heterologous expression of PKS/NRPS gene cluster and targeted anticancer drug delivery.” PCT/IB2010/055923

MARESCA, Marcello; ERLER, Axel, Steffen; FU, Jun; SEIBERT, Philipp, Martin; STEWART, Adrian, Francis; ZHANG, Youming “Method of nucleic acid recombination” PCT/IB2009/000488

ZHANG, Youming; M?LLER, Rolf; STEWART, Francis; GROSS, Frank; WENZEL,Silke, C.; FU, Jun “Methods for heterologous expression of secondary metabolites” PCT/IB2005 /003650; EP1809749 (2007); US20090298117 (2009)

张友明、王海龙、符军、A.F.斯图尔特 (03.2017)基因组大片段直接克隆和DNA多分子组装新技术,中国发明专利申请号:CN201710177676.1

张友明、尹佳、符军、郑文韬(05.2017)假单胞菌中新型重组系统及其应用,中国发明专利申请号:CN201710313101.8

张友明、尹佳、符军、姜婵娟 (03.2017)一株减毒高产鼠李糖脂的工程菌株及其构建与应用,中国发明专利申请号:CN201710172680.9

张友明、王海龙、符军、宋超逸(11.2018) 一种含有多杀菌素多操纵子人工基因簇质粒的构建方法与应用,中国发明专利申请号:CN201811407515.8