海洋微生物技术

李平一

副研究员
硕士生导师
0532-58632590
个人简介 科研项目 学术论文 其他介绍

教育背景

博士2006-2011 山东大学 微生物学

学士2002-2006 山东大学 生物技术


工作经历

2018.06-今 山东大学微生物技术国家重点实验室 副研究员

2016.07-2018.05 山东大学海洋研究院副研究员

2012.02-2015.12 山东大学化学与化工学院博士后


研究方向

1.海洋来源有机碳降解酶的挖掘、功能及结构分析

2.海洋中难培养微生物的富集培养及生理与遗传特征分析

科研项目

1. 国家自然科学基金重大研究计划培育项目,91751101,磷脂酶PlcP降解细菌膜脂的分子机制及其在海洋碳磷循环中的作用,2018.01-2020.12

2. 国家自然科学基金面上项目,41676180,含OsmC结构域的新型双结构域酯酶的催化机制及其生理功能研究,2017.01-2020.12

3. 中国博士后科学基金面上资助,2013M53158,通过构建BAC文库研究未培养MCG古菌的碳源和能量代谢,2013.08-2015.08

代表性论文

1.Yue Wang1,Ping-Yi Li1, Yi Zhang, Hai-Yan Cao, Yan-Jun Wang, Chun-Yang Li, Peng Wang, Hai-Nan Su, Yin Chen, Xiu-Lan Chen*, Yu-Zhong Zhang*.3,6-anhydro-L-galactose dehydrogenase VvAHGD is a member of a new aldehyde dehydrogenase family and catalyzes by a novel mechanism with conformational switch of two catalytic residues cysteine 282 and glutamate 248. Journal of Molecular Biology, 2020, doi: 10.1016/j.jmb.2020.02.008

2.Ping-Yi Li1, Yan-Qi Zhang1, Yi Zhang1, Wen-Xin Jiang, Yan-Jun Wang, Yi-Shuo Zhang, Zhong-Zhi Sun, Chun-Yang Li, Yu-Zhong Zhang, Mei Shi, Xiao-Yan Song,Long-Sheng Zhao*, Xiu-Lan Chen*.Study on a novel cold-active and halotolerant monoacylglycerol lipase widespread in marine bacteria reveals a new group of bacterial monoacylglycerol lipases containing unusual C(A/S)HSMG catalytic motifs.Frontiers in Microbiology, 2020, 11:9

3.Yan-Jun Wang1, Wen-Xin Jiang, Yi-Shuo Zhang, Hai-Yan Cao, Yi Zhang,Xiu-Lan Chen, Chun-Yang Li, Peng Wang, Yu-Zhong Zhang, Xiao-Yan Song*,Ping-Yi Li*. Structural insight into chitindegradation and thermostability of anovel endochitinase from theglycoside hydrolase family 18. Frontiers in Microbiology, 2019, 10:2457

4. Xiu-Lan Chen1, Fang Zhao1, Yong-Sheng Yue, Xi-Ying Zhang, Yu-Zhong Zhang,Ping-Yi Li*.A new group of modular xylanases in glycoside hydrolasefamily 8 from marine bacteria. Applied and Environmental Microbiology, 2018, 84: e01785-18

5.Ping-Yi Li1, Qiong-Qiong Yao1, Peng Wang, Yi Zhang, Yue Li, Yan-Qi Zhang, Jie Hao, Bai-Cheng Zhou, Xiu-Lan Chen*, Mei Shi, Yu-Zhong Zhang, Xi-Ying Zhang*. A novel subfamily esterase with a homoserine transacetylase-like fold but no transferase activity. Applied and Environmental Microbiology, 2017, 83:e00131~17

6.Ping-Yi Li1, Yi Zhang1, Bin-Bin Xie*, Yan-Qi Zhang, Jie Hao, Yue Wang, Peng Wang,Chun-Yang Li, Qi-Long Qin, Xi-Ying Zhang, Hai-Nan Su, Mei Shi, Yu-Zhong Zhang, Xiu-Lan Chen*. Structural andmechanisticinsights into theimprovement of thehalotolerance of amarinemicrobialesterase byincreasingintra- andinterdomainhydrophobicinteractions. Applied and Environmental Microbiology, 2017, 83: e01286~17

7. Yi Zhang1, Jie Hao, Yan-Qi Zhang, Xiu-Lan Chen, Bin-Bin Xie, Mei Shi, Bai-Cheng Zhou, Yu-Zhong Zhang,Ping-Yi Li*. Identification and characterization of a novel salt-tolerant esterase from the deep-sea sediment of the South China Sea, Frontiers in Microbiology, 2017, 8: 441

8. Fei Xu1, Fang Dong, Peng Wang, Hai-Yan Cao,Chun-Yang Li,Ping-Yi Li, Xiu-Hua Pang, Yu-Zhong Zhang, Xiu-Lan Chen*. Novel molecular insights into the catalytic mechanism of marine bacterial alginate lyase AlyGC from polysaccharide lyase family 6.Journal of Biological Chemistry, 2017, 292: 4457~4468

9.Ping-Yi Li1, Xiu-Lan Chen, Peng Ji, Chun-Yang Li, Peng Wang, Yi Zhang, Bin-Bin Xie, Qi-Long Qin, Hai-Nan Su, Bai-Cheng Zhou, Yu-Zhong Zhang, Xi-Ying Zhang*. Interdomain hydrophobic interactions modulate the thermostability of microbial esterases from the hormone-sensitive lipase family, Journal of Biological Chemistry, 2015, 290(17): 11188~11198

10.Ping-Yi Li1, Peng Ji, Chun-Yang Li, Yi Zhang, Guang-Long Wang, Xi-Ying Zhang, Bin-Bin Xie, Qi-Long Qin, Xiu-Lan Chen, Bai-Cheng Zhou, Yu-Zhong Zhang*. Structural basis for dimerization and catalysis of a novel esterase from the GTSAG motif subfamily of the bacterial hormone-sensitive lipase family, Journal of Biological Chemistry, 2014, 289(27): 19031~19041

11. Bing Guo1,Ping-Yi Li1, Yong-Sheng Yue, Hui-Lin Zhao, Sheng Dong, Xiao-Yan Song, Cai-Yun Sun, Wei-Xin Zhang, Xiu-Lan Chen, Xi-Ying Zhang*. Bai-Cheng Zhou, Yu-Zhong Zhang, Gene cloning, expression and characterization of a novel xylanase from the marine bacterium,Glaciecola mesophilaKMM241, Marine Drugs, 2013, 11(4): 1173~1187

12.Ping-Yi Li1, Bin-Bin Xie*, Xi-Ying Zhang, Qi-Long Qin, Hong-Yue Dang, Xu-Min Wang, Xiu-Lan Chen, Jun Yu, Yu-Zhong Zhang*. Genetic structure of three fosmid-fragments encoding 16S rRNA genes of the Miscellaneous Crenarchaeotic Group (MCG): implications for physiology and evolution of marine sedimentary archaea, Environmental Microbiology, 2012, 14(2): 467~479

所获专利

1.一种海洋适冷酯酶及其编码基因E40与应用,ZL201510025698.7,第二位。

2.一种海洋酯酶及其编码基因E32与应用,ZL201510216087.0,第二位。