工业微生物技术

王霞

教授
博士/硕士生导师
13075343592
个人简介 科研项目 学术论文 其他介绍

教育背景

博士:2004/09-2008/07,山东大学,发酵工程

硕士:1998/09-2001/01,无锡轻工业大学,发酵工程

学士:1994/09-1998/07,山东轻工业学院,发酵工程


工作经历

2014/09-至今,山东大学,微生物技术国家重点实验室,教授

2013/02-2014/02,美国华盛顿州立大学,分子生物科学学院,访问学者

2008/09-2014/08,山东大学,微生物技术国家重点实验室,副教授

2003/09-2008/09,山东大学,微生物技术国家重点实验室,讲师

2001/07-2003/09,山东大学,微生物技术国家重点实验室,助教


研究方向

1.环境污染物生物修复及其资源化利用

将纳米技术应用于环境污染物的生物修复及资源化利用,挖掘新型电活性微生物用于构建微生物燃料电池,实现环境污染物降解与同步产生电能;并从电化学、分子生物学等角度探究电活性微生物的胞外电子传递机制。

2.基于纳米材料生物酶固定化和生物传感器构建

基于纳米材料和生物识别元件的协同作用开发医学诊断、环境污染物监测及食品安全检测中核酸、代谢物和蛋白质等不同水平标志物的传感检测技术。

科研项目

1. 2021.01-2024.12,一株鞘氨醇杆菌的胞外电子传递机制研究,国家自然科学基金面上项目。

2.2020.01-2023.12,近海沉积物中硫氧化主要菌群与关键通路的研究(子课题:硫化物检测传感器构建),国家自然科学基金重大研发计划。

3. 2021.01-2024.12,生物反应器监控系统重构与工业生物过程优化(子课题),国家重点研发计划“合成生物学”专项。

4. 2019.01-2020.12,基于还原氧化石墨烯/纳米多孔金修饰的微生物传感器对硫化物的灵敏性检测研究,山东省科技发展计划项目。

5.2017.01-2019.12,微网过滤生物反应器工艺特性及作用机制研究,山东大学交叉学科培育项目。

6.2016.01-2019.12,基于硫醌氧化还原酶表达的微生物传感器构建及其对环境污染物硫化物检测研究,国家自然科学基金面上项目。

7.2014.01-2016.12,基于纳米多孔金辣根过氧化物酶传感器构建及其对废水中芳香族污染物检测技术开发,山东省科技发展计划项目。

8. 2012.01-2015.12,微生物与纳米材料组装复合体降解有机污染物的研究,国家自然科学基金面上项目。

9. 2010.10-2013.10,纳米颗粒与微生物复合体降解六价铬的研究,山东省优秀中青年科学家科研奖励基金。

10. 2006.12-2010.12,谷氨酸棒杆菌的代谢工程与蛋白组学研究,国家高技术研究发展计划(863计划)子课题。

11. 2008.01-2008.12,发酵法生产结冷胶多糖结构动力学研究,国家自然科学基金青年科学基金项目。

代表性论文

1. Wang Shuangjue, Chen Siyu, Shang Keshuai, Gao Xinyu,Wang Xia*.Sensitive electrochemical detection of cholesterol using a portable paper sensor based on the synergistic effect of cholesterol oxidase and nanoporous gold.International Journal of Biological Macromolecules, 2021, 189(8): 356-362.

2.XiaoSa, Wang Shuangjue,Wang Xia*, Xu Ping*. Nanoporousgold: areview andpotentials inbiotechnological andbiomedicalapplications.Nano Select, 2021,2021,2(8): 1437-1458.

3.Zhang Xueli, Wang Huimin, Xia Tian,Wang Xia*. Characterization of a new electrochemically active bacterium phylogenetically related toAlicyclobacillus hesperidum and its electrochemical performance in microbial fuel cell.Biosens. Bioelectron.,2021, 175(3): 112865.

4. Bian Congcong, Wang Huimin, Zhang Xueli, Xiao Sa, Liu Zhuang,Wang Xia*. Sensitive detection of low-concentration sulfide based on the synergistic effect of rGO, np-Au, and recombinant microbial cell.Biosens. Bioelectron., 2020, 151(3): 111985.

5. Xia Tian, Zhang Xueli, Wang Huimin, Zhang Yachao, Gao Yan, Bian Congcong,Wang Xia*, Xu Ping. Power generation and microbial community analysis in microbial fuel cells: A promising system to treat organic acid fermentation wastewater.Bioresour. Technol., 2019, 284(7): 72-79.

6. Zhang Yachao, Gao Yan, Zhang Xueli, Wang Huiming, Xia Tian, Bian Congcong, Liang Shuang, Tang Xiaozhi,Wang Xia*. Electrochemical immunosensor for HBe antigen detection based on a signal amplification strategy: The co-catalysis of horseradish peroxidase and nanoporous gold.Sens. Actuators B Chem., 2019, 284(4): 296-304.

7. Liu Zhuang, Zhang Yachao, Bian Congcong, Xia Tian, Gao Yan, Zhang Xueli, Wang Huimin, Ma Hanyue, Hu Yuan,Wang Xia*.Highly sensitivemicrobial biosensor based on recombinantEscherichia coli overexpressing catechol 2,3-dioxygenase for reliable detection of catechol.Biosens. Bioelectron., 2019, 126(2), 51-58.

8. Ma Hanyue, Xia Tian, Bian Congcong, Sun Huihui, Liu Zhuang, Wu Chao,Wang Xia*, Xu Ping.Bacterial electroactivity and viability depends on the carbon nanotube-coated sponge anode used in a microbial fuel cell.Bioelectrochemistry, 2018, 122(8): 26-31.

9. Liu Zhuang, Ma Hanyue, Sun Huihui, Gao Rui, Liu Honglei,Wang Xia*, Xu Ping, Xun Luying. Nanoporous gold-based microbial biosensor for direct determination of sulfide.Biosens. Bioelectron., 2017, 98(12), 29-35.

10. Sun Huihui, Ma Hanyue, Liu Zhuang, Li Yufei, Xu Ping,Wang Xia*. Effect of Fe3O4 nanoparticles onSphingobium yanoikuyae XLDN2-5 cells in carbazole biodegradation.Nanotechnol. Environ. Eng., 2017, 2(12): 5.

11. Sun Huihui, Liu Zhuang, Wu Chao, Xu Ping,Wang Xia*. Amperometric inhibitive biosensor based on horseradish peroxidase-nanoporous gold for sulfide determination.Sci. Rep., 2016, 6(9), 30905.

12.Wu Chao, Liu Zhuang, Sun Huihui,Wang Xia*, Ping Xu. Selective determination of phenols and aromatic amines based onhorseradish peroxidase-nanoporous gold co-catalytic strategy.Biosens. Bioelectron., 2016, 79(3), 843-849.

13. Wu Chao, Sun Huihui, Li Yufei, Liu Xueying, Du Xiaoyu,Wang Xia*, Xu Ping. Biosensor based on glucose oxidase-nanoporous gold co-catalysis for glucose detection. Biosens. Bioelectron., 2015, 66(4), 350-355.

14. Wu Chao, Liu Xueying, Li Yufei, Du Xiaoyu,Wang Xia*, Xu Ping. Lipase-nanoporous gold biocomposite modified electrode for reliable detection of triglycerides.Biosens. Bioelectron., 2014, 53(3), 26-30.

15.Liu Xueying, Du Xiaoyu,Wang Xia*, Li Naiqiang, Xu Ping, Ding Yi. Improved microbial fuel cell performance by encapsulating microbial cells with a nickel-coated sponge.Biosens. Bioelectron.,2013, 41(3), 848-851.

16.Du Xiaoyu, Liu Xueying,Li Yufei,Wu Chao,Wang Xia*, Xu Ping. Efficient biocatalyst by encapsulating lipase into nanoporous gold.Nanoscale Res. Lett., 2013, 8(1), 180-185.

17. Li Yufei, Du Xiaoyu, Wu Chao, Liu Xueying,Wang Xia*, Xu Ping. An efficient magnetically modified microbial cell biocomposite for carbazole biodegradation.Nanoscale. Res. Lett., 2013, 8(1), 522-526.

18.Wang Xia, Liu Xueying, Yan Xiuling, Zhao Peng, Ding Yi*, Xu Ping*. Enzyme-Nanoporous gold biocomposite: excellent biocatalyst with improved biocatalytic performance and stability.PLoS One, 2011, 6(9), e24207.

19.Wang Xia, Liu Xueying, Zhao Chuanming, Ding Yi*, Xu Ping*. Biodieselproduction in packed-bed reactors using lipase-nanoparticle biocomposite. Bioresour. Technol., 2011, 102(10), 6352-6355.

20.Wang Xia, Dou Peipei, Zhao Peng, Zhao Chuanming, Ding Yi*, Xu Ping*. Immobilization of lipases onto magnetic Fe3O4 nanoparticles for application in biodiesel production.ChemSusChem, 2009, 2(10), 947-950.

21.Wang Xia, Zhao Chuanming, Zhao Peng, Dou Peipei, Ding Yi, Xu Ping*.Gellan gel beads containing magnetic nanoparticles: An effective biosorbent for the removal of heavy metals from aqueous system.Bioresour. Technol., 2009, 100(7), 2301-2304.

22.Wang Xia, Gai Zhonghui, Yu Bo,Feng Jinhui, Xu Changyong, Yuan Yong, Lin Zhixin, Xu Ping*.Degradation of carbazole by microbial cells immobilized in magnetic gellan gum gel beads.Appl. Environ. Microbiol., 2007, 73(20), 6421-6428.

23.Wang Xia, Yuan Yong, Wang Kainan, Zhang Dezhong, Yang Zhengting, Xu Ping*. Deproteinization of gellan gum produced bySphingomonas paucimobilis ATCC 31461.J. Biotechnol., 2007, 128(2), 403-407.

24.Wang Xia,Xu Ping*, Yuan Yong, Liu Changlong, Zhang Dezhong, Yang Zhengting, Yang Chunyu, Ma Cuiqing.Modeling for gellan gum production bySphingomonas paucimobilis ATCC 31461 in a simplified medium.Appl. Environ. Microbiol., 2006, 72(5), 3367-3374.

获奖情况

1. 王霞,2017年山东省优秀硕士学位论文指导奖

2. 王霞,2017年山东大学优秀硕士学位论文指导奖

3. 王霞,2009年山东省优秀博士学位论文

4. 王霞,2010年全国百篇优秀博士学位论文提名奖论文


所获专利

1.一种基于溶血素O的单增李斯特菌检测试纸条及其制备与应用,中国发明专利,专利号:ZL202010037065.9

2.一种基于检测核酸hly与乙偶姻的双功能传感器检测单增李斯特菌的方法,中国发明专利,专利号:ZL202010294110.9

3. 一种利用纳米多孔金辣根过氧化物酶传感器快速检测废水中芳香族污染物的方法,中国发明专利,专利号:ZL201510888274.3

4. 一株高产结冷胶的少动鞘氨醇单孢菌及其应用,中国发明专利,专利号:ZL200610068788.5

5. 一种结冷胶发酵液的脱蛋白方法,中国发明专利,专利号:ZL200610068787.0