工业微生物技术

赵广

教授
博士/硕士生导师
个人简介 科研项目 学术论文 其他介绍

教育背景

博士:2002.09-2007.06 南开大学微生物学

学士:1998.09-2002.07 南开大学微生物学

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工作经历

2020.02至今山东大学微生物技术国家重点实验室教授

2011.05-2020.01 中国科学院青岛生物能源与过程研究所研究员

2007.09-2011.04 亚利桑那州立大学博士后


研究方向

1.细菌代谢调控

2.细菌抗逆性机制

3.生物基材料与化学品合成

科研项目

1.国家自然科学基金优秀青年科学基金-细菌抗逆性机制研究(31722001)主持2018-01至2020-12

2.山东省自然科学杰出青年基金-微生物代谢工程(JQ201707)主持2017-08至2020-08

3.中科院百人计划项目-3-羟基丙酸及其聚合物的生物合成(Y310011105)主持2013-01至2015-12

4.中科院重点部署项目课题-工业用粮对粮食安全的调控研究(ZDBS-SSW-DQC002-03)主持2017-01至2018-12

5.中科院重点部署项目课题-乙(丙)酰辅酶A羧化酶改造与3HP合成(ZDRW-ZS-2016-3M)主持2016-01至2018-12

6.中科院科技创新交叉与合作团队-生物基化学品绿色转化(Y41101105)主持2014-01至2016-12

7.中科院知识创新工程重要项目-3-羟基丙酸生物炼制的研究(KSCX2-EW-G-13) 主持 2011-01至2014-12

8.国家自然科学基金面上项目-3-羟基丙酸生物合成关键酶—丙二酸单酰辅酶A还原酶的催化机制研究和功能改造(31670089)主持 2017-01至2020-12

9.国家自然科学基金青年基金项目-大肠杆菌对 3-羟基丙酸的耐受机制研究(31200030)主持2013-01至2015-12

10.山东省自然科学基金重点项目-新型生物降解塑料聚3-羟基丙酸的生物合成(ZR2013EMZ002)主持2013-10至2016-10

代表性论文

1.XuY?, Zhao Z?, Tong WH, Ding YM, Liu B, Shi YX, Wang JC, Sun SM, Liu M, Wang YH, Qi QS, Xian M*,Zhao G*. An acid-tolerance response system protecting exponentially growingEscherichia coli.Nat Commun, 2020, 11:1496.DOI:10.1038/s41467-020-15350-5.

2.Liu M, Fu YX, Gao WJ, Xian M*,Zhao G*. Highly efficient biosynthesis of hypoxanthine inEscherichia coliand transcriptome-based analysis of the purine metabolism.ACS Synth Biol, 2020, 9(3):525-35. DOI:10.1021/acssynbio. 9b00396.

3.Zhao Z, Xian M, Liu M*,Zhao G*. Biochemical routes for uptake and conversion of xylose by microorganisms.Biotechnol Biofuels, 2020, 13:21. DOI:10.1186/s13068-020-1662-x.

4.Feng XJ, Gao WJ, Zhou YF, Zhao ZQ, Liu XT, Han XJ, Xian M*,Zhao G*. Coupled biosynthesis and esterification of 1,2,4-butanetriol to simplify its separation from fermentation broth.Eng Life Sci, 2019, 19:444-451.DOI:10.1002/elsc. 201800131.

5.Liu M, Ding YM, Xian M*,Zhao G*. Metabolic engineering of a xylose pathways for biotechnological production of glycolate inEscherichia coli.Microb Cell Fact, 2018, 17:51. DOI:10.1186/s12934-018-0900-4.

6.Feng XJ?, Jiang LQ?, Han XJ, Liu XT, Zhao ZQ, Liu HZ, Xian M*,Zhao G*. Production of D-lactate from glucose usingKlebsiella pneumoniaemutants.Microb Cell Fact, 2017, 16: 209. DOI:10.1186/s12934-017-0822-6.

7.Lactama ST, Kuiate JR, Ding YM, Xian M, Liu HZ, Boudjeko T, Feng XJ*,Zhao G*. Enhanced poly(3-hydroxypropionate) production via β-alanine pathway in recombinantEscherichia coli.PLoS One, 2017, 12:e0173150. DOI:10.1371/journal.pone.0173150.

8.Liu CS, Ding YM, Xian M, Liu M, Liu HZ, Ma QJ,Zhao G*. Malonyl-CoA pathway: a promising route for 3-hydroxypropionate biosynthesis.Crit Rev Biotechnol, 2017, 37(7):933-41. DOI:10.1080/07388551.2016. 1272093.

9.Cheng T?, Liu H?, Zou HB*, Cheng NN, Shi MX, Xie CX,Zhao G*, Xian M*. Enzymatic process optimization for thein vitroproduction of isoprene from mevalonate.Microb Cell Fact, 2017, 16:8. DOI:10.1186/s12934-016-0622-4.

10.Liu M, Ding YM, Chen HL, Zhao Z, Liu HZ, Xian M*,Zhao G*. Improving the production of acetyl-CoA-derived chemicals inEscherichia coliBL21(DE3) throughiclRandarcAdeletion.BMC Microbiol, 2017, 17:10. DOI:10.1186/s12866-016-0913-2.

11.Tong WH, Xu Y, Xian M, Niu W, Guo JT, Liu HZ*,Zhao G*. Biosynthetic pathway for acrylic acid from glycerol in recombinant Escherichia coli.Appl Mircobiol Biotechnol, 2016, 100(11): 4901-7. DOI:10.1007/s00253-015-7272-z.

12.Liu CS, Ding YM, Zhang RB, Liu HZ, Xian M*,Zhao G*. Functional balance between enzymes in malonyl-CoA pathway for 3-hydroxypropionate biosynthesis.Metab Eng, 2016, 34: 104-11. DOI:10.1016/j.ymben.2016.01.001.

13.Liu M, Yao L, Xian M, Ding YM, Liu HZ*,Zhao G*. Deletion ofarcAincreased the production of acetyl-CoA-derived chemicals in recombinantEscherichia coli.Biotechnol Lett, 2016, 38(1):97-101. DOI:10.1007/s10529-015-1953-7.

14.Feng XJ, Ding YM, Xian M, Xu X, Zhang RB,Zhao G*. Production of optically pure D-lactate from glycerol by engineeredKlebsiella pneumoniaestrain.Bioresour Technol, 2014, 172:269-75. DOI:10.1016/j.biortech. 2014.09.074.

15.Wang Q, Yang P, Xian M, Feng L, Wang JM,Zhao G*.Metabolic engineering ofEscherichia colifor poly (3-hydroxypropionate) production from glycerol and glucose.Biotechnol Lett, 2014, 36(11):2257-62. DOI:10.1007/s10529-014-1600-8.

16.Gao YQ, Liu CS, Ding YM, Sun C, Zhang RB, Xian M*,Zhao G*. Development of genetically stableEscherichia colistrains for poly(3-hydroxypropionate) production.PLoS One, 2014, 9(5): e97845. DOI:10.1371/journal.pone.0097845.

17.Wang Q, Yang P, Xian M, Liu H, Cao YJ, Yang Y,Zhao G*.Production of block copolymer poly(3-hydroxybutyrate)-block-poly(3-hydroxypropionate) with adjustable structure from inexpensive carbon source.ACS Macro Lett, 2013, 2(11): 996-1000.DOI:10.1021/mz400446g.

18.Liu CS, Wang Q, Xian M, Ding YM,Zhao G*. Dissection of malonyl-coenzyme A reductase ofChloroflexus aurantiacusresults in enzyme activity improvement.PLoS One, 2013, 8(9): e75554. DOI:10.1371/journal.pone.0075554.

19.Gao YQ?, Feng XJ?, Xian M, Wang Q,Zhao G*. Inducible cell lysis systems in microbial production of bio-based chemicals.Appl Microbiol Biotechnol, 2013, 97(16): 7121-9. DOI:10.1007/s00253-013-5100-x.

20.Wang Q, Yang P, Xian M, Yang Y, Liu CS, Xue YC,Zhao G*. Biosynthesis of Poly(3-hydroxypropionate-co-3-hydroxybutyrate) with fully controllable structures from glycerol.Bioresour Technol, 2013, 142: 741-4. DOI:10.1016/j.biortech.2013.05.121.

21.Wang Q, Yang P, Liu CS, Xue YC, Xian M*,Zhao G*. Biosynthesis of poly(3-hydroxypropionate) from glycerol by recombinantEscherichia coli.Bioresour Technol, 2013, 131: 548-51. DOI:10.1016/j.biortech.2013.01.096.

22.Wang Q, Liu CS, Xian M, Zhang YG,Zhao G*. Biosynthetic pathway for poly(3-hydroxypropionate) in recombinantEscherichia coli.J Microbiol, 2012, 50(4): 693-7. DOI:10.1007/s12275-012-2234-y.

23.Zheng YN, Li LL, Liu Q, Yang JM, Wang X, Liu W, Xu X, Liu H,Zhao G*, Xian M*. Optimization of fatty alcohol biosynthesis pathway for selectively enhanced production of C12/14 and C16/18 fatty alcohols in engineeredEscherichia coli.Microb Cell Fact, 2012, 11: 65. DOI:10.1186/1475-2859-11-65.

24.Zhao G?, Kong W?, Weatherspoon N, Clark-Curtiss J, Shi Y*. Mg2+facilitates leader peptide translation to induce riboswitch-mediated transcription termination.EMBO J, 2011, 30(8): 1485-96. DOI:10.1038/emboj.2011.66. (?Contributed equally to this work)

25.Weatherspoon N?,Zhao G?, Kong W, Kong Y, Morigen, Andrews-Polymenis H, McClelland M, Shi Y*. The CpxR/CpxA two-component system upregulates two Tat-dependent peptidoglycan amidases to confer bacterial resistance to antimicrobial peptide protamine.J Biol Chem, 2011, 286(7): 5529-39. DOI:10.1074/jbc.M110.200352. (?Contributed equally to this work)

26.Zhao G, Weatherspoon N, Kong W, Curtiss R 3rd, Shi Y*. A dual-signal regulatory circuit activates transcription of a set of divergent operons inSalmonella typhimurium.P Natl Acad Sci USA,2008, 105(52): 20924-9. DOI:10.1073/pnas.0807071106.

获奖情况

2017 国家基金委优青

2017 山东省杰青

2012 中国科学院百人计划

2007 天津市自然科学一等奖

2007 中国高校十大科技进展


所获专利

1.赵广、冯新军、咸漠、刘修涛、刘会洲、赵志强。一种生产3-羟基丙酸共聚物的重组菌及其构建方法和应用。申请号:201710291725.4。(授权日期2019.11.12)

2.赵广、冯新军、韩晓娟、咸漠。一种利用葡萄糖合成D-乳酸的重组菌及其构建方法与应用。申请号:201610851557.5。(授权日期2019.09.23)

3.赵广、姚岚、冯新军、咸漠、刘会洲。一种通过镁离子诱导大肠杆菌裂解的重组载体及应用。申请号:201610224643.3。(授权专利2019.09.04)

4.咸漠、童文华、赵广、刘会洲。一种利用甘油生产丙烯酸的重组菌及其构建方法与应用。专利号:ZL201510514412.1。(授权日期2018.11.30)

5.咸漠、韩雪萍、赵广。一种3-羟基丙酸耐受性提高的大肠杆菌及其构建方法。专利号:ZL201510175613.3。(授权日期2018.11.13)

6.咸漠、唐勇、赵广、廖本仁、冯新军、揭元萍、刘会洲、张春雷。一种催化甲醇制备异戊二烯的大肠杆菌基因工程菌及其制备方法与应用。专利号:ZL201510771229.X。(授权日期2018.09.18)

7.咸漠、赵广、刘敏、曹玉锦、刘长水。一种高产间苯三酚的基因工程菌及其构建方法与应用。专利号:ZL201410722836.2。(授权日期2018.05.04)

8.咸漠、赵广、刘敏。一种高产间苯三酚基因工程菌的构建方法及应用。专利号:ZL201510459220.5。(授权日期2018.05.04)

9.咸漠、赵广、冯新军。一种聚3-羟基丙酸共聚物及其生产方法。专利号:ZL201410272402.7。(授权日期2017.02.15)

10.咸漠、冯新军、赵广、张汝兵。一种联产3-HP和P3HP的重组肺炎克雷伯氏菌及其制备方法和应用。专利号:ZL201310409148.6。(授权日期2015.01.07)