资源与环境微生物技术

倪金凤

教授
博士/硕士生导师
053258631564
个人简介 科研项目 学术论文 其他介绍

教育背景

博士 1998.04-2002.03日本岐阜大学生物资源科学应用微生物学

硕士 1988.09-1991.06兰州大学细胞生物学细胞工程

学士1984.09-1988.06兰州大学生物系动物学


工作经历

2009.12-至今 山东大学微生物技术国家重点实验室 教授/博导

2012.09-2013.08韩国首尔大学生化工程系访问教授

2004.09-2006.09日本农业生物资源研究所 日本学术振兴会特别研究员

2004.04-2009.11山东大学微生物技术国家重点实验室副教授/硕导

2002.05-2004.03日本农业生物资源研究所 博士后

1996.09-1997.01英国伯明翰大学生物系实习研究

1991.07-1997.10 兰州军区总医院科研训练课 主管技师


研究方向

1.极端微生物基因组编辑与合成生物学研究

2.耐热、耐酸微生物底盘细胞的设计与构建

3.特殊环境资源微生物由来活性天然产物的挖掘与利用


科研项目

1.2020/07-2025/6,国家重点研发计划“合成生物学重点专项”(2020YF0906802)极端微生物底盘细胞的设计与构建—课题2:耐热、耐酸微生物底盘细胞的设计构建与表征)子课题主持

2.2020/01-2024/12,国家自然科学基金面上项目:黄翅大白蚁抗菌活性放线菌发掘与抗真菌化合物鉴定,主持

3.2015/01-2018/12,国家自然科学基金面上项目:冰岛硫化叶菌膜泡转运蛋白复合体III同源蛋白的遗传分析,主持

4.2013/01-2016/12,国家自然科学基金面上项目:黄翅大白蚁新型内源性木质纤维素降解酶性质与功能分析,主持

5.2011/01-2015/12,国家重点基础研究发展(973)计划子课题:木质纤维素生物降解多样性、新降解因子及相关机理的子课题,子子课题主持

6.2010/01-2016/12,国家重点实验室课题:培菌白蚁木质纤维素降解机制研究,主持

7.2009/10-2011/12,国家自然科学基金面上项目:白蚁后肠共生微生物由来新木质纤维降解酶基因的筛选与鉴定,主持

8.2008/01-2010/12,国家自然科学基金面上项目:利用硫化叶菌异源表达超嗜热古菌重组蛋白的研究,国家自然科学基金,主持

9.2008/01-2010/12,教育部留学回国人员科研启动基金:白蚁葡萄糖苷酶的分子改造及酵母工程菌的构建,主持

10.2010/01-2013/12,国家自然科学基金重点项目:超嗜热古菌DNA重组修复解旋酶和核酸酶的作用机制及体内功能,主要参与者,排名第二

发表论文

1.Lin J, Shen Y,Ni J*, She Q*(2021) A type III-A CRISPR–Cas system mediates co-transcriptional DNA cleavage at the transcriptional bubbles in close proximity to active effectors. Nucleic Acids Research, 49(13):7628-7643.

2.J. Li, M. Sang, Y. Jiang, J. Wei, Y. Shen, Q. Huang, Y. Li,Ni J.*(2021) Polyene-producing Streptomyces spp. from the fungus-growing termiteMacrotermes barneyiexhibit high inhibitory activity against the antagonistic fungusXylaria,Front Microbiol 12.https://doi.org/10.3389/fmicb.2021.649962.

3.Y. Xiao, J. Li, P. Wu, N. Ning, J. Li, Y. Shen, Q. Huang*,Ni J.*(2021) An alkaline thermostable laccase from termite gut associated strain ofBacillus stratosphericus, Int J Biol Macromol 179: 270-278.

4.Zhang S, Lin Z, Huang Q, Shen Y,Ni J*(2021). First record of gregarine protists (Apicomplexa: Sporozoa) in Asian fungus-growing termiteMacrotermes barneyi(Blattaria: Termitidae). Sci Rep. 11(1):989.

5.Qihong Huang, Zijia Lin, Pengju Wu,Jinfeng Ni*, Yulong Shen*(2020) Phosphoproteomic analysis reveals Rio1-related protein phosphorylation changes in response to UV irradiation inSulfolobus islandicusREY15A, Front Microbiol, 11: 586025.

6.Ahmad, S., Huang, Q.,Ni J.,Xiao, Y., Yang, Y.,Shen, Y.*(2020). Functional Analysis of the NucS/EndoMS of the Hyperthermophilic ArchaeonSulfolobus islandicusREY15A. Front Microbiol11, 607431.

7.Li J, Cao C, Jiang Y, Huang Q, Shen Y*,Ni J* (2020). A Novel Digestive GH16 β-1,3(4)-Glucanase from the Fungus-Growing TermiteMacrotermes barneyi. Appl Biochem Biotechnol. 192(4):1284-1297.

8.Nagam V, Aluru R, Shoaib M, Dong GR, Li Z, Pallaval VB,Ni JF*(2020). Diversity of fungal isolates from fungus-growing termiteMacrotermes barneyiand characterization of bioactive compound fromXylaria escharoidea. Insect Sci. doi: 10.1111/1744-7917.12799. PMID: 32394613.

9.Yutong Jiang, Shuo Zhang, Zijia Lin,Jinfeng Ni* (2020) Advances in lignocellulose-degrading enzymes from termites and symbiotic microbes, Acta Microbiologica Sinica.蒋宇彤,张硕,林子佳,倪金凤*(2020)白蚁消化系统转化和降解木质纤维素酶研究进展.微生物学报2020, 60(12): 1–15.

10.Zhang Shuo, Zijia Lin, XIAO Yuan-xi,LI Jing-jing,LI Zhi,Jinfeng Ni*(2020) Analysis of symbiotic microorganism diversity in comb and feces of fungus-growing termite,Microbiology China.张硕,林子佳,肖元玺,李净净,李枝,倪金凤* (2020)培菌白蚁菌圃和粪便微生物多样性分析[J/OL].微生物学通报2020, 47(10): 3091?3101.

11.Mayaka Joseph Badys, Huang Qihong, Xiao Yuanxi, Zhong Qing,Ni Jinfeng,Shen, Yulong*(2019) The lonely guy (LOG) homologue SiRe_0427 from the thermophilic archaeonSulfolobus islandicusREY15A is a phosphoribohydrolase representing a novel group. Appl. Environ. Microbiol. 85(20).

12.Huang Qihong, Mayaka Joseph Badys, Zhong Qing, Zhang Chao, Hou Guihua,Ni Jinfeng, Yulong Shen*(2019) Phosphorylation of the archaeal Holliday junction resolvase Hjc inhibits its catalytic activity and facilitates DNA repair inSulfolobus islandicusREY15A. Frontiers in Microbiology, 10: 1214.

13.Jianhua Wei,Jingjing Li,Jinfeng Ni*(2019) Research progress of fungus-growing insects associated actinomycetes and its secondary metabolites,Acta Microbiologica Sinica,2019, 59(10): 1864-1871.未建华,李净净,倪金凤*培菌昆虫相关放线菌的次级代谢产物研究进展[J/OL].微生物学报.59(10): 1864-1871.

14.Jiao Du, Shuzhe Jiang, Jianhua Wei, Yulong Shen, andJinfeng Ni*(2019). Co-expression of lignocellulase from termite and their endosymbionts. Chinese Journal of Biotechnology, 35(2): 244?253.杜娇,姜淑喆,未建华,申玉龙,倪金凤*白蚁及其共生菌来源的4种木质纤维素酶的共表达,生物工程学报, 35(2): 244?253.

15.Zhai B, DuPrez K, Han, H, Yuan Z, Ahmad S, Xu C, Gu L,Ni J,Fan L, Yulong Shen (2018) The archaeal ATPase PINA interacts with the helicase Hjm via its carboxyl terminal KH domain remodeling and processing replication fork and Holliday junction. Nucleic Acids Research. 46(13):6627~6641.

16.Shuzhe Jiang, Jingjing Li, Chunjing Cao, Yulong Shen, andJinfeng Ni*(2018)Molecular modification of β-glucosidase from the midgut ofMacrotermes barneyi. Chinese Journal of Biotechnology, 34(7): 1081?1090.姜淑喆,李净净,曹春静,申玉龙,倪金凤* (2018)黄翅大白蚁来源β-葡萄糖苷酶的分子改造,生物工程学报, 34(7): 1081?1090。

17.Sun X, Wei J, Li J, Ni J.*(2018)Whole-genome analysis of the dominant bacteriaDysgonomonas macrotermitisin the hindgut ofMacrotermes barneyi. Acta Microbiologica Sinica, 58(6):995-1003.孙新新,未建华,李净净,倪金凤*黄翅大白蚁后肠优势菌Dysgonomonas macrotermitis的全基因组序列分析,微生物学报。

18.Sun X, Li J, Du J, Xiao H,Ni J.*(2018)Cellulomonas macrotermitissp. nov., a chitinolytic and cellulolytic bacterium isolated from the hindgut of a fungus-growing termite. Antonie Van Leeuwenhoek.2018, 111(3): 471-478.

19.Liu J, Gao R, Li C,Ni J,Yang Z, Zhang Q, Chen H, Shen Y. (2017) Functional assignment of multiple ESCRT-III homologs in cell division and budding inSulfolobus islandicus. Mol Microbiol. 105(4), 540–553.

20.Zhai B, DuPrez K, Doukov TI, Li H, Huang M, Shang G,Ni J,Gu L, Shen Y, Fan L. (2017) Structure and Function of a Novel ATPase that Interacts with Holliday Junction Resolvase Hjc and Promotes Branch Migration. J Mol Biol. 7;429(7):1009-1029.

21.Huang Q, Zhong Q, Mayaka J,Ni J, Shen Y (2017) Autophosphorylation and Cross-phosphorylation of Protein Kinases from the CrenarchaeonSulfolobus islandicus. Front Microbiol, 8: 2173.

22.Yan Z, Yuan Z,Ni J, Gu L, Shen Y. (2017) Crystal structure of the crenarchaeal ExoIII AP endonuclease SisExoIII reveals a conserved disulfide bond endowing the protein with thermostability. Biochem Biophys Res Commun. doi: 10.1016/j.bbrc.2017.06.116. PMID: 28647366

23.Li J, Sun X, andNi J.*(2017)Overview of research methods on intestinal microflora of termites. Biotic Resources, 39 (4), 247-255.李净净孙新新倪金凤*白蚁肠道微生物研究方法概述,生物资源。

24.Ning N, Tan H andNi J.*(2017)Isolation and identification of chitin-degrading bacteria from the hindgut ofMacrotermes barneyi,Microbiology China, 44 (7), 1649-54.孙新新李净净宁娜谭慧军倪金凤*黄翅大白蚁后肠几丁质降解微生物的分离与鉴定,微生物学通报。

25.Jingjing Li, Junfeng Liu, Yulong Shen andJinfeng Ni* (2017)Swionibacillus sediminis gen. nov., sp. nov., a member of family Bacillaceae isolated from Southwest Indian Ocean. International Journal of Systematic and Evolutionary Microbiology. Int J Syst Evol Microbiol. 2017, 67(9): 3440-3445.

26.Na Ning, Huijun Tan, Xinxin Sun, andJinfeng Ni*(2017) Advance of heterologous expression study of eukaryote-origin laccases,Chinese Journal of Biotechnology, 33 (4), 565-577.宁娜,谭慧军,孙新新,倪金凤*真核生物来源漆酶的异源表达研究进展,生物工程学报。

27.SUN Xinxin, Ning Na, Tan Huijun,NI Jinfeng*(2017) Research progress of diversity of symbiotic microbes in the gut of termites,Chin J Appl Environ Biol,2017, 04: 764-770.孙新新,宁娜,谭慧军,倪金凤*白蚁肠道微生物多样性和作用研究进展,应用与环境生物学报。

28.Yan Z, Huang Q,Ni J, Shen Y.(2016) Distinct catalytic activity and in vivo roles of the ExoIII and EndoIV AP endonucleases fromSulfolobus islandicus. Extremophiles. 20(5):785-93.

29.Shi X, Zhang N, Ning N, Yun C,Ni J*(2016) Isolation and identification of xylan -degrading bacteria from fungus-growing termite hindgut. Microbiology China. 3(3): 504-509.石小玉,张宁,宁娜,员超,倪金凤*(2016)一株培菌白蚁后肠由来的木聚糖降解细菌的分离与鉴定,微生物学通报。

30.Song X,Ni J, Shen Y.(2016) Structure-based genetic analysis of Hel308a in the hyperthermophilicarchaeonSulfolobus islandicus. J Genet Genomics. 43(6):405-13. PMID: 27317310

31.Song X, Huang Q,Ni J, Shen Y.(2016)Knockout and functional analysis of two DExD/H-box family helicase genes inSulfolobus islandicusREY15A. Extremophiles. 20(4):537-46.

32.Sun X, Yang Y, Zhang N, Shen Y,Ni J*. (2015) Draft Genome Sequence ofDysgonomonas macrotermitisStrain JCM 19375T,Isolated from the Gut of a Termite. Genome Announc, 3(4), 10.1128/genomeA.00963-15.

33.Ning N, Zhang N, Wu Y andNi J*. (2015) Comparison of lignocellulolytic enzyme activities of the fungus-growing termiteMacrotermes barneyiand the lower termite Coptotermes formosanus. Chin J Appl Environ Biol, 21(4): 678-82.宁娜,张倪,吴燕,倪金凤*台湾乳白蚁和黄翅大白蚁消化道主要木质纤维素降解酶活性的比较。应用与环境生物学报,21(4): 678-82。

34.Huang Q, Liu L, Liu J,Ni J,She Q and Shen Y (2015) Efficient 5′-3′DNA end resection by HerA and NurA is essential for cell viability in the crenarchaeonSulfolobus islandicus. BMC Molecular Biology 16:2 (DOI:10.1186/s12867-015-0030-z)

35.Huang Q, Li Y, Zeng C, Song T, Yan Z,Ni J,She Q, Shen Y (2015) Genetic analysis of the Holliday junction resolvases Hje and Hjc inSulfolobus islandicus. Extremophiles. 19:505-14

36.Ni J,Wu Y, Yun C, Yu ML and Shen Y. (2014) cDNA cloning and heterologous expression of an endo-β-1,4-glucanase from the fungus-growing termiteMacrotermes barneyi. Arch Insect Biochem Physiol. 86(3):151-64.

37.Yang YJ, Zhang N, Ji SQ, Lan X, Zhang KD, Shen YL, Li FL,Ni JF* (2014) Dysgonomonas macrotermitis sp. nov., isolated from the hindgut of a fungus-growing termite. Int J Syst Evol Microbiol., 64:2956-61.

38.Yun C, Ning N,Ni J*. (2014) Analysis of cellulose-degrading microbiome ofMacrotermes barneyihindgut. Bioprocess Engineering, 2014. 12(1): 13-7.员超,张宁,倪金凤*黄翅大白蚁后肠降解滤纸微生物群落的分析。生物加工工程, 2014. 12(1): 13-7。

39.Yu M,Ni J*. (2014) Research advances on the insect laccase. Bioprocess Engineering, 12(1): 80-5.于孟兰,倪金凤*昆虫漆酶的研究进展。生物加工工程, 2014. 12(1): 80-5。

40.Ni J1*, Tokuda, G. (2013) Lignocellulose–Degrading Enzymes from Termites and Their Symbiotic Microbiota. Biotechnol. Adv. 31(6):838-50.

41.Liang P, Han W, Huang Q, Li Y,Ni J, She Q Shen Y (2013) Knockouts of RecA-Like Proteins RadC1 and RadC2 Have Distinct Responses to DNA Damage Agents inSulfolobus islandicus. J Genet Genomics. 40:533-42.

42.Zheng T, Huang H, Zhang C,Ni J, She Q, Shen Y (2012) Development of simvastatin selection marker for hyperthermophilic acidophile, Appl. Environ. Microbiol. 78:568-574.

43.Wu Y, Chi S, Yun C, Shen Y, Tokuda G,Ni J*. (2012) Molecular cloning and characterization of an endogenous digestive β-glucosidase from the midgut of the fungus-growing termiteMacrotermes barneyi. Insect Mol Biol.21:604-14.

44.Hong Y, Chu M, Li Y,Ni J, Sheng D, Hou G, She Q, Shen Y (2012) Dissection of the functional domains of an archaeal Holliday junction helicase, DNA Repair. 11:102-111.

45.Wu Y, Hou XF, Chi SL andNi J. * (2012) Isolation and characterization of aBacillus cereusstrain with cellulose-degradation activity from a termite-inhabiting soil. Microbiology China. 39: 638-44.吴燕,侯信锋,迟绍丽,倪金凤*(2012)白蚁栖息环境中蜡状芽孢杆菌的分离及其纤维素酶活性分析。微生物学通报。

46.Wu Y, Chi SL andNi J. * (2011) Isolation of a strain ofVariovorax sp. R3063 with cellulase activity from termite. Chinese Journal of Applied Entomology. 48: 33-28.吴燕,迟绍丽,倪金凤*. (2011)从白蚁中分离到具有纤维素酶活的贪噬菌.应用昆虫学报, 2011, 48(1): 33-28.

47.Ni J.*, Takehara M and Watanabe H. (2010) Identification of Activity Related Amino Acid Mutations of a GH9 Termite Cellulase. Bioresource Technology. 101:6438-43.

48.Wu L., Liu, B., Hong, Y., Sheng, D., Shen, Y.,Ni J.* (2010) Residue Tyr224 is critical for the thermostability ofGeobacillussp. RD-2 lipase, Biotechnol Lett. 32:107-12.

49.Liu, B., Wu L., Liu, T., Hong, Y., Shen, Y.,Ni, J.* (2009) A MOFRL family glycerate kinase from the thermophilic crenarchaeonSulfolobus tokodaiiwith unique enzymatic properties, Biotechnol Lett. 31:1937-41.

50.Wang F, Zhang, S. Huang, Q, Shen, Y andNi, J.* (2009) Advance in genetic manipulation systems of Hyperthermophilic archaea. Acta Microbiologica Sinica 49, 1418-23.

51.Huang Q, Shen Y andNi, J.* (2008) Optimization of selectivr conditions for the selection of uracil auxotrophs of thermophilic archaeaSulfolobus tokodaii. Journal of Shandong University (Natural Science), 43, 6-10.

52.Ni J., Takehara M, Miyazawa M and Watanabe H. (2007) Random exchanges of non-conserved amino acid residues among four parental termite cellulases by family shuffling improved thermostability. Protein Eng. Des. Sel. 20, 535-42.

53.Ni J., Tokuda G, Takehara M and Watanabe H. (2007) Heterologous expression and enzymatic characterization of β-glucosidase from the drywood-eating termite,Neotermes koshunensis. Appl. Entomol. Zool. 42, 457-63.

54.Ni J., Takehara, M and Watanabe H. (2005) Heterologous overexpression of a mutant termite cellulase gene inE.coliby DNA shuffling of four orthologous parental cDNAs, Biosci. Biotechnol. Biochem. 69: 1711-20.

55.Ni J., Sasaki, Y., Tokuyama, S., Sogabe, A., and Tahara, Y. (2002) Conversion of a typical catalase from Bacillus sp. TE124 to a catalase-peroxidase by directed evolution. J. Biosci. Bioeng. 93, 31-6.

56.Ni J., Tokuyama, S., Sogabe, A., Kawamura, Y., and Tahara, Y. (2001)Cloning and high expression of catalase gene fromBacillussp. TE124. J. Biosci. Bioeng. 91, 422-4.

英文著作

Watanabe H. andNi J*. (2013) Functional gene resources from cellulose-feeding insects for novel catalyst in “Biological Conversion of Biomass for Fuels and Chemicals: Explorations from Natural biomass Utilization Systems”. RSC Energy and Environment 2013, p156-76.


授权专利

倪金凤,申玉龙,郑涛,佘群新;一种基于抗生素标记的超嗜热泉古菌表达载体及其应用, 2011-09-07,中国, CN201110020921.0.


获奖情况

日本学术振兴会(JSPS)外国人特别研究员奖励(2004-2006)

2018年度山东大学生命科学学院本科生优秀导师


学术兼职

中国 昆虫微生物组 专业委员会 委员

中国 城市昆虫 专业委员会 委员


讲授课程

《专业英语》、《极端环境微生物学》、全校核心素质通选课《微生物与人类》以及 全校通识课程 《微生物与人类生活》。


招生信息

招生专业:生物化学与分子生物学、微生物学

欢迎有志于科学研究的学子们踊跃报考。

另外课题组长期招收博士后,欢迎对木质纤维素降解利用及其合成生物学感兴趣的博士生加入课题组。