资源与环境微生物技术

赵建

教授
博士/硕士生导师
0532-58632409
个人简介 科研项目 学术论文 其他介绍

教育经历

1999年7月研究生毕业于天津科技大学,获工学博士学位,同年进入山东大学微生物技术国家重点实验室博士后流动站,2001年8月出站留校工作,9月评聘为副教授,硕士生导师;2005年破格晋升教授,博士生导师。2006.9-2007.9加拿大康考迪亚大学生物系访问学者,2014.9-2015.3美国威斯康辛大学麦迪逊校区农业与生命科学学院高级研究学者。


学术兼职:

中国微生物学会环境微生物专业委员会委员

中国发酵工业协会理事

工业酶产业技术创新战略联盟学术委员会委员

黑龙江省寒区环境微生物与农业废弃物资源化利用重点实验室学术委员会委员


科研方向

1木质纤维素的生物降解和生物转化

(1)木质纤维素降解酶(如纤维素酶、半纤维素酶、木质素降解酶类、阿魏酸酯酶等)优良菌株的分子生物学改造

(2)酶的发酵生产及其应用;

(3)木质纤维素不同组分的高效分离和转化利用技术(如生产纤维素乙醇、木糖醇、木质素产品等)。其中纤维素乙醇、木糖醇等已实现工业化规模生产。

2碱性/耐碱性酶类的研发及其应用

适合造纸、纺织、印染、食品等工业不同工艺要求的碱性/耐碱性酶制剂的研发和生产,例如碱性/耐碱性纤维素酶、碱性漆酶、碱性果胶酶等的高产菌株的工程改造和选育、工业用复合酶系研发、生产及其应用等。其中造纸工业用酶和碱性果胶酶已经成功应用于工业化生产。

3部分农副产品的深加工技术

如玉米、生姜等农产品中有效成分的分离提取、残渣的加工利用等

主持项目

先后主持包括6项国家自然科学基金,2项国家863计划、国家973计划、国际合作项目、多项省级重大重点项目等在内的各类项目20多项,以及与中粮集团、中国石油等多家企业合作的多个横向课题。近年来部分课题如下:

?国家自然科学基金面上项目:纤维素酶在木质素上的吸附规律和机制及弱木质素吸附性酶的构建, 2019-2022

?山东省重点研发计划(重大科技创新工程)项目:杜仲木质纤维素高值化利用关键技术,2019-2021

?中石油合作横向课题:高效纤维素酶菌株构建及发酵产酶技术研究,2018-2023

?山东省自然科学基金(重大基础类):木质纤维素类生物质高效降解转化的关键机理研究,2019-2024

?山东省重点研发计划:甜高粱秆固体发酵残渣生物转化生产液体燃料乙醇及其综合利用研究,2019-2021

?国家自然科学基金面上项目:木质纤维素材料预处理过程中木质素结构和性质的变化及其对纤维素酶吸附的影响及机制,2013-2016

?山东省自主创新重大专项:造纸行业生物质主流组分分离与高值利用关键技术研究与示范,2014-2017

?国家973计划(2011CB707401):生物质预处理及对纤维素酶解与转化的影响机制,2011-2015

?国家863计划(2012AA022203B):造纸用木质素酶和纤维素酶的分子改造及应用,2012-2015

研究成果

以第一或通讯作者在国内外发表研究论文100多篇,其中SCI、EI等收录80多篇;已授权国家发明专利11件;参与编写著作3部,译著1部。

近年来发表的部分文章:

1 Tiantian Yang, Yingjie Guo, Na Gao, Xuezhi Li, Jian Zhao*.Modification of a cellulase system by engineering Penicillium oxalicum to produce cellulose nanocrystal,Carbohydrate Polymers, 2020, Vol 234, 115862 IF 7.182 (一区)

2 Tiantian Yang, Xuezhi Li, Yingjie Guo, Shengjuan Peng, Guodong Liu, Jian Zhao*.Effect of endoglucanases from different glycoside hydrolase families on enzymatic preparation of cellulose nanocrystal. Industrial Crops and Products,155(2020): 112755 IF4.244(一区)

3 Daolei Zhang, Xuezhi Li*, Jian Zhao*.Fusarium concolor?X4 pretreatment suppresses light-induced yellowing of high-yield pulp.BioMed Research International, Volume 2020, Article ID 9497215

4 Fenfen Guo,Xuezhi Li*, Jian Zhao*, Guanxi Li, Peike Gao, Xiaolong Han. Optimizing Culture Conditions by Statistical Approach to Enhance Production of Pectinase fromBacillus?sp. Y1. BioMed Research International. Volume 2019, Article ID 8146948

5 Yanan Li; Yueyan Hu; Zhu Zhu; Kaili Zhao; Guodong Liu; Lushan Wang; Yinbo Qu; Jian Zhao; Yuqi Qin*. Normal transcription of cellulolytic enzyme genes relies on the balance between the methylation of H3K36 and H3K4 inPenicillium oxalicum.Biotechnology for Biofuels, 2019, 12:198 (JCR一区),IF 6.343

6Ya'nan Li, Yueyan Hu, Kaili Zhao, Yunjun Pan, Yinbo Qu, Jian Zhao, Yuqi Qin*. The indispensable role of histone methyltransferase PoDot1 in extracellular glycoside hydrolase biosynthesis ofPenicillium oxalicum, Frontiers in Microbiology, 2019, 10: 2566(JCR一区) IF 4.840

7 Xianqin Lu, Xiaoting Feng, Xuezhi Li, Jian Zhao*. Binding and hydrolysis properties of engineered cellobiohydrolases and endoglucanases. Bioresource Technology, 2018. 267:235-241(一区)IF6.669

8 Xianqin Lu, Xiaoting Feng, Xuezhi Li, Jian Zhao*. The adsorption properties of endoglucanase to lignin and their impact on hydrolysis, Bioresource Technology, 2018. 267:110-116(一区)IF6.669

9 Can Wang, Xianqin Lu, Jia Gao, Xuezhi Li, Jian Zhao*. Xylo-oligosaccharides Inhibit Enzymatic Hydrolysis by Influencing Enzymatic Activity of Cellulase from Penicillium oxalicum, Energy & Fuels, 2018, 32:9427-9437 (二区)

10 Jian Du, Wenxia Song, Xiu Zhang, Jian Zhao, Guodong Liu, Yinbo Qu*, Differential Reinforcement of enzymatic hydrolysis by adding chemicals and accessory proteins to high solid loading substrates with different pretreatments. Bioprocess and Biosystems Engineering 2018, 41:1153-1163

11 Jian Du, Yuan Cao, Guodong Liu, Jian Zhao, Xuezhi Li, Yinbo Qu*. Identifying and overcoming the effect of mass transfer limitation on decreased yield in enzymatic hydrolysis of lignocellulose at high solid concentrations. Bioresource Technology, 2017,229:88-95(一区)IF5.807

12 Meimei Wang,·Yanxin Ye,·Xuezhi Li,·Hao Chen,·Jian Zhao*, Utility of co-expressed alkali-tolerant endoglucanase and xylanase in ameliorating wheat straw chemical pulp properties, Cellulose, 2017, 24(5), 2299-2311 (一区)

13Yanxin Ye, Xuezhi Li, Yuan Cao, Jian Du, Shicheng Chen, Jian Zhao*.A β-xylosidase hyper-production Penicillium oxalicum mutant enhanced ethanol production from alkali-pretreated corn stover. Bioresource Technology, 2017,245:734-742 (一区)IF5.807

14Yanxin Ye, Xuezhi Li, Jian Zhao*.Production and Characteristics of a Novel Xylose- and Alkali-tolerant GH 43 β-xylosidase fromPenicillium oxalicum for Promoting Hemicellulose Degradation. Scientific Reports, 2017, 7:11600 (二区)

15 Xianqin Lu, Can Wang, Xuezhi Li, Jian Zhao*. Temperature and pH influence adsorption of cellobiohydrolase onto lignin by changing the protein properties. Bioresource Technology, 2017,245:819-825 (一区) IF5.807

16 Meimei Wang, Jian Du, Daolei Zhang, Xuezhi Li, Jian Zhao*.Modification of Different Pulps by Homologous Overexpression Alkali-Tolerant Endoglucanase inBacillus subtilis Y106, Scientific Reports, 2017, 7:3321(二区)

17 Xianqin Lu, Can Wang, Xuezhi Li, Jian Zhao*, Xuebing Zhao.Studying nonproductive adsorption ability and binding approach of cellobiohydrolase to lignin during bioconversion of lignocellulose, Energy & Fuels, 2017,31 (12): 14393–14400,2017-10-19(二区)

18Liping Tan, Meimei Wang, Xuezhi Li, Hongxing Li,Jian Zhao*,Yinbo Qu, Yuen May Choo, Soh Kheang Loh. Fractionation of oil palm empty fruit bunch by bisulfite pretreatment for the production of bioethanol and high value products, Bioresource Technology, 2016, 200: 572-578 (一区) IF 5.651

19 Xianqin Lu,XiaojuZheng,Xuezhi Li, Jian Zhao*.Adsorption and mechanism of cellulase enzymes onto lignin isolated from corn stover pretreated with liquid hot water, Biotechnology for Biofuels, 2016, 9:118-129 (JCR一区),IF7.398 (5-years)

20 Daolei Zhang, Xuezhi Li*, Meimei Wang, Yanxin Ye, Jian Du, Xianqin Lu, Jian Zhao*. Xylanase Treatment Suppresses Light- and Heat-Induced Yellowing of Pulp, Scientific Reports, 2016,6:38374,(二区),IF5.525(5-years)

21 Yanan Li, Xiaoju Zheng, Xiujun Zhang, Longfei Bao, Yingying Zhu, Yinbo Qu, Jian Zhao*, Yuqi Qin*. The Different Roles of Penicillium oxalicum LaeA in the Production of Extracellular Cellulase andβ-xylosidase, Frontiers in Microbiology, 2016,7: 2091 (JCR一区),IF 4.165

奖励:

2014年 山东省科技进步二等奖

2014年 山东省优秀硕士学位论文指导奖

2014年 山东大学优秀硕士学位论文指导奖

2004年 山东省科学技术发明二等奖

2004年 山东高等学校优秀科研成果二等奖(自然科学类)

指导的多名博士生和硕士生连续多年获得国家奖学金、优秀毕业生等。