医药微生物技术

马莉

副研究员
硕士生导师
0532-58632496
个人简介 科研项目 学术论文 其他介绍

教育背景

博士 起止时间:2009.09-2013.01,毕业院校:中国科学院微生物研究所,专业:生物化学与分子生物学

硕士 起止时间:2006.09-2009.06,毕业院校:中国海洋大学,专业:遗传学

学士 起止时间:2002.09-2006.06,毕业院校:山西农业大学,专业:生物工程


工作经历

起止时间 单位名称 专业技术职务

2022.03至今,微生物技术国家重点实验室,山东大学,副研究员

2019.03-2022.02,微生物技术国家重点实验室,山东大学,助理研究员

2013.07-2019.02,中国科学院青岛生物能源与过程研究所,助理研究员

2013.01-2013.06,国家海洋局第一海洋研究所,研究助理


研究方向

1.酶工程,包括:工业酶资源的筛选、蛋白质进化改造、450酶的结构功能研究

2.生物催化

科研项目

1. 2021.01-2024.12,P450cam和P450BM3共进化同工酶的结构功能关系解析,国家自然科学基金面上项目,NSFC 32071266,主持;

2. 2019.12-2024.11,合成塑料高效生物降解的关键技术—课题2:合成塑料降解酶晶体结构及催化机制解析,国家重点研发计划“变革性技术关键科学问题”重点专项2019YFA0706900,骨干;

3. 2019.09-2021.12,细胞色素P450酶的理性设计,山东大学基本科研业务费,2019GN030,主持;

4. 2017.05-2019.05,重组大肠杆菌全细胞高效生产γ-羟基-N-甲基-L-亮氨酸4-环孢菌素A的关键技术研究,青岛市应用基础研究计划项目,17-1-1-60-jch,主持;

5. 2016.01-2019.12,P450单加氧酶AveE催化阿维菌素呋喃环形成的机制研究,国家自然科学基金青年基金项目,NSFC 31600045,主持;

6. 2016.11-2019.06,利用重组大肠杆菌全细胞催化系统高效生产毛发生长促进剂的关键技术研究,山东省自然科学基金青年基金项目,ZR2016CQ05,主持。

代表性论文

1.Ma L, Li F, Zhang X, Chen H, Huang Q, Su J, Liu X, Sun T, Fang B, Liu K, Tang D, Wu D, Zhang W, Du L, Li S*. Development of MEMS directed evolution strategy for multiplied throughput and convergent evolution of cytochrome P450 enzymes. Sci China Life Sci,2022,65(3): 550-560. (IF: 6.038)

2.Guo J, Li F, Cheng F,Ma L, Liu X, Durairaj P, Zhang G, Tang D, Long X, Zhang W, Du L, Zhang X*, Li S. Bacterial biosynthetic P450 enzyme PikCD50N: a potential biocatalyst for the preparation of human drug metabolites. J Org Chem, 2021, 86(21): 14563-14571. (IF: 4.354)

3.You C, Li F, Zhang X,Ma L, Zhang YZ, Zhang W, Li S*. Structural basis for substrate speci?city of the peroxisomal acyl-CoA hydrolase MpaH’involved in mycophenolic acid biosynthesis. FEBS J, 2021, 288, 5768-5780.(IF: 5.542)

4.Li F,Ma L, Zhang X, Chen J, Qi F, Huang Y, Qu Z, Yao L, Zhang W, Kim ES, Li S.Structure-guided manipulation of the regioselectivity of the cyclosporine A hydroxylase CYP-sb21 fromSebekia benihana.Synth Syst Biotechnol, 2020, 5(3): 236-243. (IF: 4.708)

5.Dong S, Chen J, Zhang X, Guo F,Ma L, You C, Wang X, Zhang W, Wan X, Liu SJ, Yao LS, Li S, Du L*, Feng Y*. Structural basis for selective oxidation of phosphorylated ethylphenolsby cytochrome P450 monooxygenase CreJ. Appl Environ Microbiol, 2021, 87: e00018-21. (IF: 4.792)

6.Li Z, Jiang Y, Zhang X, Chang Y, Li S, Zhang X, Zheng S, Geng C, Meng P,Ma L, Yang Y, Gao Z, Tang YJ, Li S*. Fragrant venezuelaenes A and B with a 5?5?6?7 tetracyclic skeleton: discovery, biosynthesis, and mechanisms of central catalysts.ACS catalysis, 2020, 10, 5846-5851. (IF: 13.084)

7.Li Z#, Jiang Y#, Guengerich FP,Ma L, Li S, Zhang W*. Engineering cytochrome P450 enzyme systems for biomedical and biotechnological applications.J Biol Chem. 2020, 295(3): 833-849. (IF: 5.517)

8.Xu H, Liang W, Ning L, Jiang Y, Yang W, Wang C, Qi F,Ma L, Du L, Fourage L, Zhou Y J, Li S*. Directed evolution of P450 fatty acid decarboxylases via high-throughput screening towards improved catalytic activity. ChemCatChem, 2019, 11, 1-6.(IF: 5.686)

9.Yao Q#,Ma L#, Liu L, Ikeda H, Fushinobu S, Li S, Xu LH*. Hydroxylation of Compactin (ML-236B) by CYP105D7 (SAV_7469) fromStreptomyces avermitilis. J Microbiol Biotechnol, 2017, 27(5): 956-964. (IF: 2.351)

10.Sun Y#,Ma L#, Han D#, Du L, Qi F, Zhang W, Sun J, Huang S, Kim ES*, Li S*.In vitroreconstitution of the cyclosporine specific P450 hydroxylases using heterologous redox partner proteins. J Ind Microbiol Biotechnol, 2017, 44(2):161-166.(IF: 3.346)

11.Du L,Ma L, Qi F, Zheng X, Jiang C, Li A, Wan X, Liu SJ*, Li S*. Characterization of a unique pathway for 4-cresol catabolism initiated by phosphorylation inCorynebacterium glutamicum. J Biol Chem, 2016, 291(12): 6583-6594.(IF:5.157)

12.Ma L, Du L, Chen H, Sun Y, Huang S, Zheng X, Kim ES, Li S*. Reconstitution of thein vitroactivity of the cyclosporine-specific P450 hydroxylase fromSebekia benihanaand development of a heterologous whole cell biotransformation system. Appl Environ Microbiol. 2015, 81(18), 6268-6275.(IF: 4.792)

13.Li C#,Ma L#, Mou S, Wang Y, Zheng Z, Liu F, Qi X, An M, Chen H, Miao J*. Cyclobutane Pyrimidine Dimers Photolyase from Extremophilic Microalga: Remarkable UVB Resistance and Efficient DNA Damage Repair, Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis, 2015, 773: 37-42. (IF: 3.68)

14.Liu X#, Song F#,Ma L, Chen C, Xiao X, Ren B, Liu X, Dai H,Piggott AM, Av-Gay Y, Zhang L*, Capon RJ*. Sydowiols A–C:Mycobacterium tuberculosisprotein tyrosine phosphatase inhibitors from an East China Sea marine-derived fungus,Aspergillus sydowii. Tetrahedron Letters, 2013, 54(45):6081-6083. (IF: 2.415)

15.Ma L, Liu X, Liang H, Che Y, Chen C, Dai H, Yu K, Liu M, Ma LY, Yang CH, Song F, Wang Y*, Zhang L*. Molecular targets of 14-alpha-lipoyl andrographolide on quorum sensing inPseudomonas aeruginosa. Antimicrob Agents Chemother, 2012, 56(12): 6088-6094.(IF: 5.191)

16.Zeng X, Liu X, Bian J, Pei G, Dai H, Polyak SW, Song F,Ma L, Wang Y*, Zhang L*. Synergistic effect of AL-1 and various antibiotics on the formation of biofilms and production of exopolysaccharide and pyocyanin byPseudomonas aeruginosa.Antimicrobial Agents and Chemotherapy, 2011, 55(6): 3015-3017. (IF: 5.191)

17.Ma L, Chen J*, Liu R, Zhang XH, Jiang YA.Mutation ofrpoSgene decreased resistance to environmental stresses, synthesis of extracellular products and virulence ofVibrio anguillarum.FEMS Microbiol Ecol, 2009, 70(2): 130-136.(IF:4.194)

所获专利

李盛英,马莉,Eung-Soo Kim。一种制备毛发生长促进剂的生物转化方法(申请号:201510346350.8,申请公布号:CN106319007A,授权号:ZL201510346350.8)

缪锦来,许建方,马莉。一种南极冰藻CPD光修复酶、其编码基因和表达载体以及该酶的应用(申请号:201310121837.7,申请公布号:CN103160488A,授权号:ZL201310121837.7)