医药微生物技术

王明钰

教授
博士/硕士生导师
0532-58630856
个人简介 科研项目 学术论文 其他介绍

教育背景

博士 起止时间:2003.08-2010.10毕业院校:宾夕法尼亚州立大学 专业:生物化学、微生物学与分子生物学

学士 起止时间:1999.09-2003.07毕业院校:北京大学 专业:生物科学


工作经历

起止时间 单位名称 专业技术职务

2019.09--今 山东大学? 教授

2013.09--2019.08 山东大学 副教授

2011.07-2013.08 山东大学 讲师

2010.10-2011.04 宾夕法尼亚州立大学 博士后


研究方向

1.临床及环境耐药性机制与调查;

2. 耐药性及其转移的分子调控机制;

3. 体内体外微生物组模型;

4. 微生物感染相关技术开发

科研项目

1.山东大学基本科研业务费交叉学科培育项目“医疗废水中耐药性威胁的消除及其机制研究”,No. 2018JC013,2018年1月-2020年12月,参与,25万元

2.山东大学基本科研业务费交叉学科培育项目“地球大气层中抗生素耐药基因的分布与传播机制研究”,No. 2018JC027,2018年1月-2020年12月,主持,25万元

3.微生物技术国家重点实验室开放课题“口腔溃疡的微生物学基础”,2018年7月-2019年5月,No. M2018-07,主持,3万元

4.横向课题“体育器材及场所的耐药菌污染”,2018年4月-2020年4月,主持,11万元

5.微生物技术国家重点实验室开放课题“Dissemination and removal of antibiotic pollution and resistance in aquatic ecosystems”,2017年5月-2018年5月,参与,2万元

6.山东省重点研发计划“口腔感染细菌类型及抗生素耐药性的快速检测技术”,2018年1月-2019年12月,参与,15万元

7.济南市文化产业发展专项资金“古籍字画霉变的防治技术”,2017年10月-2019年12月,技术负责人,20万元

8.国家重点研发计划“我国传统膳食结构的肠道微生态与健康效应相关性研究”,No. 2017YFD0400301,2017年7月-2020年12月,子课题主持人,108万元

9.国家自然科学基金面上项目“里氏木霉中诱导纤维素酶表达的碳源信号转导途径及其机制”,No. 31770043,2018年1月-2021年12月,项目主持人,55万元

10.山东大学基本科研业务费交叉学科培育项目“呼吸道病原体的抗生素耐药性及其机制研究”,No. 2017JC028,2017年1月-2019年12月,参与,35万元

11. 2016年度生命科学学院创新基金“1型整合子中抗生素耐药基因的表达调控机制”,2016年11月-2016年12月,项目主持人,8万元

12.山东省重点研发计划“高效纤维素酶制剂的理性设计、制备与应用”,No. 2016GSF121040, 2016年9月-2017年12月,项目主持人, 10万元。

13.山东大学基本科研业务费交叉学科培育项目“污水处理系统中抗生素和抗性基因的污染特征与强化去除机制”,No. 2015JC025,2015年1月-2017年12月,交叉学科负责人,35万元

14.国家科技支撑计划“秸秆等低值生物质生产高清洁汽柴油技术集成及产业化”课题中“基于木质纤维素的汽油/柴油有效组分的生物转化”,No. 2014BAD02B07,2014年1月-2016年12月,子课题主持人,80万元

15.教育部留学归国人员科研启动基金“热纤梭菌中氢酶的分布与性质研究”,2013年12月-2015年12月,项目主持人,3.5万元

16.国家自然科学基金青年基金“热纤梭菌中膜上Ech氢酶复合体的功能与性质研究”,No. 31200051,2013年1月-2015年12月,项目主持人,23万元

17.山东省自然科学基金青年基金“热纤梭菌中的Ech氢酶性质研究”,No.ZR2012CQ022,2012年7月-2015年7月,项目主持人,6万元

18.山东大学自主创新基金项目“热纤梭菌的产氢机制研究”,No. 2011HW008,2011年10月-2013年12月,项目主持人,9万元

19.山东省自然科学基金“基于WGS的多重耐药性院内传播途径鉴定与阻断技术”,2021年1月-2023年12月,No. ZR2020MH308,参与,10万元

20.山东省重点研发计划(重大科技创新工程)“新型冠状病毒现场快速联合检测技术及装备开发”,No. 2020CXGC011305,课题负责人,142.35万元

21.横向项目“基于恒温扩增的核酸检测技术开发”,主持,120万元

代表性论文

53.Li L, Zhang M, Wang W, Xia R, Ma Y, Wei X, Wang X, Sun X, Xie X, Xie S, Wang M*, Xu H*. 2021. Identification and characterization of two novel ISCR1-associated genes dfrA42 and dfrA43 encoding trimethoprim resistant dihydrofolate reductases. Antimicrob Agents Chemother 65(6):e02010-20.

52. Li Z, Shi L, Wang B, Wei X, Zhang J, Guo T, Kong J, Wang M *, Xu H *. 2020. In vitro assessment of antimicrobial resistance dissemination dynamics during multidrug resistant bacteria invasion events using a continuous culture device. Appl Environ Microbiol doi: 10.1128/AEM.02659-20.


51. Wang M 1, Wang W 1, Niu Y, Liu T, Li L, Zhang M, Li Z, Su W, Liu F, Zhang X *, Xu H *. 2020. A clinical extensively-drug resistant (XDR) Escherichia coli and role of its β-lactamase genes. Front Microbiol 11:590357. doi:10.3389/fmib.2020.590357.


50. Yang Z 1, Cui Q1, Zhang M, Li Z, Wang M*, Hai X*. 2020. A lux-based Staphylococcus aureus bioluminescence screening assay for the detection/identification of antibiotics and prediction of antibiotic mechanisms. J Antibiot 73:828-836. doi: 10.1038/s41429-020-0349-7.


49. Yang Z, Cui Q, An R, Wang J, Song X, Shen Y, Wang M *, Xu H *. 2020. Comparison of microbiomes in ulcerative and normal mucosa of recurrent aphthous stomatitis (RAS)-affected patients. BMC Oral health 20:128. doi: 10.1186/s12903-020-01115-5.

48.Wei T, Gong J, Qu G,Wang M*, Xu H. 2019. Interactions betweenYersinia pestisV-antigen (LcrV) and human Toll-like Receptor 2 (TLR2) in a modelled protein complex and potential mechanistic insights. BMC Immunol 20:48. doi: 10.1186/s12865-019-0329-5.

47.Yu T, Kong J, Zhang L, Gu X,Wang M, Guo T*. 2019. New crosstalk between probioticsLactobacillus plantarumandBacillus subtilis. Sci Rep 9(1):13151. doi: 10.1038/s41598-019-49688-8.

46.Wang F1,Wang M1, Zhao Q, Niu K, Liu S, He D, Liu Y, Xu S, Fang X*. 2019. Exploring the relationship betweenClostridium thermocellumJN4 andThermoanaerobacterium thermosaccharolyticumGD17. Front Microbiol 10: 2035. doi: 10.3389/fmicb.2019.02035

45.Li L, Yu T, Ma Y, Yang Z, Wang W, Song X, Shen Y, Guo T, Kong J,Wang M*, Xu H *. 2019. The genetic structures of an Extensively Drug Resistant (XDR)Klebsiella pneumoniaeand its plasmids. Front Cell Infect Microbiol 8: 446.

44.Wang M1, Ma Y1, Li L, Wang B, Wei X, Zhang M, Wang J, Cui Q, Li Z *, Xu H *. 2019. The diversity of glycosylation of cellobiohydrolase I fromTrichoderma reeseidetermined with mass spectrometry. Biochem Biophys Res Commun 508(3): 818-824.

43.Bie L, Fang M, Li Z,Wang M*, Xu H *. 2018. Identification and characterization of new resistance-conferring SGI1s (Salmonella genomic island 1) inProteus mirabilis. Front Microbiol 9: 3172. doi: 10.3389/fmicb.2018.03172.

42.Wan X, Gao M, Wang Y, Xu H,Wang M, Wang X*. 2017. Formation, characteristics and microbial community of aerobic granular sludge in the presence of sulfadiazine at environmentally relevant concentrations. Bioresour Technol250:486-494.

41.Yuan S, Gao M, Zhu F, Afzal MZ, Wang Y, Xu H,Wang M, Wang S, Wang X*. 2017. Disintegration of aerobic granules during prolonged operation. Environ Sci: Water Res Technol3:757.

40.Wang M1, Shen W1, Yan L, Wang X, Xu H*. 2017. Stepwise impact of urban wastewater treatment on bacterial community structure, content of antibiotics, and prevalence of antimicrobial resistance. Environ Pollut 231(P2):1578-1585.

39.李紫云,王明钰,徐海*.细菌II型、III型整合子在耐药性传播中的作用[J].中国抗生素杂志, 2018,43(2):156-162.

38.崔庆宇,王明钰,徐海*.lux荧光报告系统的研究进展[J].中国生物工程杂志, 2017,37(8):66-71.

37.Wang M, Zhang M, Li L, Dong Y, Jiang Y, Liu K, Zhang R, Jiang B, Niu K, Fang X*. 2017. Role ofTrichoderma reeseimitogen-activated protein kinases (MAPKs) in cellulase formation. Biotechnol Biofuels10:99.

36.Guo N, Wang Y*, Yan L, Wang X,Wang M, Xu H, Wang S*. 2017. Effect of bio-electrochemical system on the fate and proliferation of antibiotic resistance genes during the treatment of chloramphenicol wastewater. Water Res117:95-101.

35.马亚楠,王明钰*,徐海.纤维素酶糖基化的研究进展[J].微生物学报, 2017,57(8):1-8.

34.Bie L, Wu H, Wang X,Wang M*, Xu H*. 2017. Identification and Characterization of new SXT/R391 family integrative and conjugative elements (ICEs) inProteus mirabilisisolates from broiler carcasses. Int J Antimicrob Agents50(2):242-246

33.Yan Z,Wang M, Ferry JG*. 2016. A Ferredoxin- and F420H2-dependent, electron-bifurcating, heterodisulfide reductase with homologs in the domainsBacteriaandArchaea. mBio8:e02285-16.

32.Yan L, Liu D, Wang X, Wang Y,Wang M*, Xu H*. 2017. Bacterial plasmid-mediated quinolone resistance genes in aquatic environments in China. Sci Rep7:40610.

31.Wang M*, Hou J*. 2017. Biorefinery of lignocellulosics for biofuels and biochemicals, p 143-191.InLau PCK (ed), Quality living through chemurgy and green chemistry, Springer-Verlag GamH, Germany.

30.Wu H,Wang M,Liu Y, Wang X, Wang Y, Lu J, Xu H*. 2016. Data on the phylogenetic typing, integron gene cassette array analysis, multi-drug resistance analysis and correlation between antimicrobial resistance determinants inKlebsiellastrains. Data Brief8:1289-1294.

29.Wang M, Zhao Q, Li L, Niu K, Li Y, Wang F, Jiang B, Liu K, Jiang Y, Fang X*. 2016. Contributing factors in the improvements of cellulosic H2production inClostridium thermocellum/Thermoanaerobacteriumco-cultures. Appl Microbiol Biotechnol100(19):8607-8620.

28.Liu R, Wang X*, Zhang Y,Wang M, Gao M, Wang S. 2016. Optimization of operation conditions for the mitigation of nitrous oxide (N2O) emissions from aerobic nitrifying granular sludge system. Environ Sci Pollut Res23(10):9518-9528.

27.沈伟涛,王明钰,王允坤,王新华,徐海*.抗生素含量测定方法的分析综述[J].中国生物工程杂志, 2016, 36(6): 119-126.

26.Wu H,Wang M, Liu Y, Wang X, Wang Y, Lu J, Xu H*. 2016. Characterization of antimicrobial resistance inKlebsiellaspecies isolated from chicken broilers. Int J Food Microbiol232:95-102.

25.Gao M, Yang S,Wang M, Wang X*. 2016. Nitrous oxide emissions from an aerobic granular sludge system treating low-strength ammonium wastewater. J Biosci Bioeng doi: 10.1016/j.jbiosc.2016.04.004.

24.Jiang B, Zhang R, Feng D, Wang F, Liu K, Jiang Y, Niu K, Yuan Q,Wang M,Wang H, Zhang Y, Fang X*. 2016. A Tet-on and Cre-loxP based genetic engineering system for convenient recycling of selection markers inPenicillium oxalicum. Front Microbiol7:486.

23.Wang F, Jiang Y, Guo W, Niu K, Zhang R, Hou S,Wang M, Yi Y, Zhu C, Jia C, Fang X*. 2016. An environmentally friendly and productive process for bioethanol production from potato waste. Biotechnol Biofuels9:50.

22.Liu K, Dong Y, Wang F, Jiang B,Wang M,Fang X*. 2016. Regulation of cellulase expression, sporulation and morphogenesis by velvet family proteins inTrichoderma reesei. Appl Microbiol Technol100(2):769-779.

21.Wang M,Han L, Liu S, Zhao X, Yang J, Loh SK, Sun X, Zhang C, Fang X*. 2015.A Weibull statistics-based lignocellulose saccharification model and a built-in parameter accurately predict lignocellulose hydrolysis performance. Biotechnol J10(9):1424-1433.

20.Wang F, Liu K, Han L, Jiang B,Wang M, Fang X*. 2015.Function of a p24 heterodimer in morphogenesis and protein transport inPenicillium oxalicum. Sci Rep5:11875.

19.Wang M, Yang H, Zhang M, Liu K, Wang H, Luo Y, Fang X*. 2015.Functional analysis ofTrichoderma reeseiCKIIα2, a catalytic subunit of casein kinase II.Appl Microbiol Biotechnol99(14):5929-5938.

?18.Wang M,Dong Y, Zhao Q, Wang F, Liu K, Jiang B, Fang X*. 2014.Identification of the role of a MAP kinase Tmk2 inHypocrea jecorina(Trichoderma reesei). Sci Rep 4:6732.

17.张杰,王明钰,张晓东,方诩*,李红震.以木质纤维素原料生产生物丁醇的研究进展[J].生物产业技术, 2014, 2014(7月): 59-64.

16.Suharti S,Wang M, de Vries S, Ferry JG*. 2014.Characterization of the RnfB and RnfG Subunits of the Rnf Complex from the ArchaeonMethanosarcina acetivorans. PLoS ONE9(5):e97966.

15.王方忠,蒋艺,刘奎美,姜宝杰,王明钰,方诩*.丝状真菌中纤维素酶与半纤维素酶的合成调控[J].生物加工过程, 2014, 12(1): 72-79.

14.王明钰,沈煜,徐孝刚,邵明,魏天迪,方诩*.山东大学生物类拔尖人才培养模式的探索[J].高校生物学教学研究(电子版), 2013, 3(4): 3-6.

13.Wang F, Liang Y,Wang M, Yang H, Liu K, Zhao Q, Fang X*. 2013.Functional diversity of the p24γ homologue Erp reveals physiological differences between two filamentous fungi. Fungal Genet Biol61:15-22.

12.自振滔,石文昊,李钰茜,翟贞文,韩丽娟,王明钰*,方诩*.利用等离子诱变技术改造纤维素酶生产丝状真菌工业菌株[J].中国酿造, 2013, 32(S1): 5-8.

11.Wang M,He D, Liang Y, Liu K, Jiang B, Wang F, Hou S, Fang X*. 2013.Factors involved in the response to improvement of agitation during cellulase production fromPenicillium decumbensJUA10-1. J Ind Microbiol Biotechnol40(9):1077-1082.

10.Wang M,Zhao Q, Yang J, Jiang B, Wang F, Liu K, Fang X*. 2013.A mitogen-activated protein kinase Tmk3 participates in high osmolarity resistance, cell wall integrity maintenance and cellulase production regulation inTrichoderma reesei. PLoS ONE8(8): e72189.

9.Wang M,Mu Z, Wang J, Hou S, Han L, Dong Y, Xiao L, Xia R, Fang X*. 2013.The identification of and relief from Fe3+inhibition for both cellulose and cellulase in cellulose saccharification catalyzed by cellulases fromPenicillium decumbens. Bioresour Technol133:507-512.

8.Wang M, Liu K, Dai L, Zhang J, Fang X*. 2013.The structural and biochemical basis for cellulose biodegradation. J Chem Technol Biot88(4):491-500.

7.Zhang J,Wang M, Gao M, Fang X*, Yano S, Qin S, Xia R. 2013.Efficient acetone-butanol-ethanol production from corncob with a new pretreatment technology - wet disk milling. Bioenerg Res6(1):35-43.

6.周广麒,李晶晶,李忠海,吕晶,王明钰,曲音波,方诩*.斜卧青霉转录调控因子BglR的缺失对纤维素酶生产的影响[J].微生物学通报, 2012, 39(10): 1379-1387.

5.周广麒,吕晶,李忠海,李晶晶,王明钰,曲音波,肖林,覃树林,赵海涛,夏蕊蕊,方诩*.斜卧青霉去泛素化蛋白酶CREB的缺失提高纤维素酶的生产[J].生物工程学报, 2012, 28(8): 959-972.

4.王涫,王明钰,穆子铭,侯少莉,伏小平,方诩*.里氏木霉纤维素酶生产工艺的优化[J].黑龙江农业科学, 2012, 2012(5): 108-111.

3.Wang M,Li Z, Fang X*, Wang L, Qu Y. 2012. Cellulolytic enzyme production and enzymatic hydrolysis for second-generation biofuel production. Adv Biochem Eng Biotechnol128:1-24.

2.Wang M,Tomb JF, Ferry JG*. 2011.Electron transport in acetate-grownMethanosarcina acetivorans. BMC Microbiol11:165.

1.Doerfert SN1, Reichlen M1, Iyer P,Wang M,Ferry JG*. 2009.Methanolobus zinderi sp. nov.,a methylotrophic methanogen isolated from a deep subsurface coal seam. Int J Syst Evol Microbiol59(Pt 5):1064-1069.

所获专利

王明钰, 赵秋爽,方诩, 候少丽. 一种真菌纤维素酶酶系组成/特性调控基因及其应用: 中国,201310142896.2[P].