医药微生物技术

颜富

教授
博士/硕士生导师
个人简介 科研项目 学术论文

教育背景

博士 2012.10-2017.3 德国萨尔大学/亥姆霍兹药物研究所 药物生物技术

2008.9-2012.10湖南师范大学 微生物学

学士 2004.10-2008.6湖南科技大学 生物技术


工作经历

起止时间 单位名称 专业技术职务

2019.9-至今 山东大学 微生物技术研究院/国家重点实验室 教授

2019.5-2019.8山东大学微生物技术研究院/国家重点实验室 副研究员

2017.1-2019.3德国萨尔州亥姆霍兹药物研究所 博士后


研究方向

1.微生物活性代谢产物的挖掘与生物合成机制研究

2. 活性天然产物的合成生物学改造

3.噬菌体基因组编辑与耐药菌治疗

科研项目

1. 国家重点研发计划“绿色生物制造”重点专项青年科学家项目,工业微生物基因组大片段DNA的设计原理与操纵方法,2021-2024,项目骨干

2. 国家自然科学基金青年基金(82003639),厌氧梭菌活性天然产物的发现及生物合成机制研究, 2021-2023,主持

3. 国家重点研发计划“合成生物学”重点专项子课题(2019YFA0904003),基因组重编程实现噬菌体精准控制,2020-2024,主持

4. 山东省泰山学者青年专家计划,2020-2024,主持

5. 山东大学齐鲁青年学者启动经费,2019-2024, 主持

代表性论文

Liu Yang, Zhou Haibo, Shen Qiyao, Dai Guangzhi,Yan Fu, Li Xiaoju, Ren Xiangmei, Sun Qin, Tang Ya-Jie , Zhang Youming, Bian Xiaoying*. Discovery of Polycyclic Macrolide Shuangdaolides by Heterologous Expression of a Cryptic trans-AT PKS Gene Cluster.Organic Letters. 2021, DIO: 10.1021/acs.orglett.1c02589.

李瑞娟,赵晓雨,杨润雨,刘洋,颜富,王海龙,张友明,符军*.噬菌体重组酶介导的DNA 同源重组工程.微生物学通报. 2021, DOI: 10.13344/j.microbiol.china.210402.

Chen Hanna, Sun Tao, Bai Xianping, Yang Jie,Yan Fu, Yu Lei, Tu Qiang, Li Aiying, Tang Yajie, Zhang Youming,* Bian Xiaoying * and Zhou Haibo*. Genomics-driven activation of silent biosynthetic gene clusters inBurkholderia gladioliby screening recombineering system.Molecules, 2021, 26, 700.

Yan Fuand Müller Rolf*.Class I methyltransferase VioH catalyzes unusual SAM cyclization leading to 4-methylazetidinecarboxylic acid formation in vioprolide biosynthesis.ACS Chemical Biology, 2019, 14(1), 99–105.

Yan Fu,# Auerbach David,# Chai Yi, Keller Lena, Tu Qiang, Hüttel Stephan, Glemser Ameli, Grab A. Hanusch, Bach Thorsten, Zhang Youming,* Müller Rolf*. Biosynthesis and heterologous production of vioprolides: rational biosynthetic engineering and unprecedented 4-methylazetidinecarboxylic acid formation.Angewandt Chemie International Edition, 2018, 57(28):8754-8759.

Yan Fu,# Burgard Christian,# Popoff Alexander, Zaburannyi Nestor, Zipf Gregor, Maier Josef, Bernauer S. Hubert, Wenzel C. Silke, Müller Rolf*.Synthetic biology approaches and combinatorial biosynthesis towards heterologous lipopeptide production.Chemical Science, 2018, 9, 7510-7519.

Auerbach David,#Yan Fu,# Zhang Youming and Müller Rolf*. Characterization of an unusual glycerate esterification process in vioprolide biosynthesis.ACS Chemical Biology, 2018, 13(11), 3123–3130 .

Bian Xiaoying, Plaza Alberto,Yan Fu, Zhang Youming, Müller Rolf*. Rational and efficient site-directed mutagenesis of adenylation domain alters relative yields of luminmide.Biotechnology and bioengineering, 2015, 112(7):1343-1353.

Yin Jia, Hoffmann Michael, Bian Xiaoying, Tu Qiang,Yan Fu, Xia Liqiu, Ding Xuezhi, Stewart A. Francis, Müller Rolf, Fu Jun, Zhang Youming*. Direct cloning and heterologous expression of the salinomycin biosynthetic gene cluster fromStreptomyces albusDSM41398 inStreptomyces coelicolorA3(2).Scientific reports, 2015, 5, 15081.

Yan Fu, Cheng Xing, Ding Xuezhi, Yao Ting, Chen Hanna, Li Wenping, Hu Shengbiao, Yu Ziquan, Sun Yunjun, Zhang Youming, Xia Liqiu*. Improved insecticidal toxicity by fusing Cry1Ac ofBacillus thuringiensiswith Av3 ofAnemonia viridis.Current Microbiology, 2014, 68(5):604-609.

梁毅偈, 孙运军, 胡胜标,颜富,张旭, 白利明, 张友明, 丁学知, 夏立秋*. 大肠杆菌Nissle1917 L-天冬酰胺酶Ⅱ基因在大肠杆菌BL21中的表达与抗肿瘤活性.微生物学通报, 2014, 41(4): 607?613.

Zhang Yunlei, Zhang Youming, Xia Liqiu,* Zhang Xiangli, Ding Xuezhi,Yan Fu, Wu Feng.E.coliNissle 1917 targets and cures the primary tumors through expressing azurin protein.Applied Environmental Microbiology, 2012, 78(21):7603-7610.

Li Wenping, Xia Liqiu,* Ding Xuezhi, Lv Yuan, Luo Yushuang, Hu Shengbiao, Yin Jia,Yan Fu. Expression and characterization of a recombinant Cry1Ac crystal protein fused with an insect-specific neurotoxin ω-ACTX-Hv1a inBacillus thuringiensis.Gene, 2012, 498(2):323-327.