医药微生物技术

王海龙

教授
博士/硕士生导师
个人简介 科研项目 学术论文 其他介绍

教育背景

博士 硕博连读:2006-2012毕业院校:湖南师范大学 专业:微生物学

博士 联合培养:2009-2010 德国德累斯顿工业大学 专业:分子生物学

学士 起止时间:2002-2006 毕业院校:湖南师范大学 专业:生物基地班

???

工作经历

起止时间 单位名称 专业技术职务

2018-至今, 山东大学 微生物技术国家重点实验室 教授

2013-2017, 山东大学 微生物技术国家重点实验室 博士后


研究方向

1.微生物合成生物学

2.基因编辑技术

3.微生物药物生物合成

科研项目

1.国家自然科学基金优秀青年基金:微生物基因编辑与药物合成生物学(32122049),2022.1-2024.12,主持

2.山东省泰山学者青年专家,2019.1-2023.12,主持

3.山东大学齐鲁青年学者,2018.7-2023.6,主持

4.国家重点研发计划子课题(2019YFA0904001),非天然噬菌体的设计合成,2020.1-2024.12,主持。

5.国家重点研发计划子课题(2018YFA0900403),放线菌底盘适配性机理与产物高产机制研究,2019.7-2024.6,主持。

6.山东省自然科学基金重大基础研究项目(ZR2019ZD22),高效表达非洲猪瘟病毒多抗原的伪狂犬载体构建及评价,2019.12-2024.12,主持。

7.国家自然科学基金青年项目(31700073),植物促生土著链霉菌的人工改良以增强其杀线虫活性,2018.01?2020.12,主持,已结题。

8.中国博士后科学基金特别资助项目(2015T80710),改造埃博霉素生物合成途径以提高单一组分的产量和纯度,2015.07-2017.01,主持,已结题。

9.山东省博士后创新项目(201303110),通过诱发细胞内DNA双链断裂来直接克隆基因组DNA,2013.10-2015.06,主持,已结题。

代表性论文

1.Song, C.#; Luan, J.#; Li, R.; Jiang, C.; Hou, Y.; Cui, Q.; Cui, T.; Tan, L.; Ma, Z.; Tang, Y. J.; Stewart, A. F.; Fu, J.; Zhang, Y. &Wang, H.*(独立通讯作者), RedEx: a method for seamless DNA insertion and deletion in large multimodular polyketide synthase gene clusters,Nucleic Acids Research, 2020, 48(22), e130.(IF=16.971,生化与分子生物学一区)

2.Jiang, C; Zhou, H; Sun, H; He, R; Song, C; Cui, T; Luan, J; Fu, J; Zhang, Y; Jiao, N;Wang, H.*(独立通讯作者),Establishing an efficient salinomycin biosynthetic pathway in three heterologousStreptomyceshosts by constructing a 106-kb multioperon artificial gene cluster,Biotechnology & Bioengineering, 2021, in press. (IF=4.530,生物工程与应用微生物二区)

3.Abbasi, M. N.; Fu, J.; Bian, X.;Wang, H.*(并列通讯作者); Zhang, Y.* & Li, A.*, Recombineering for Genetic Engineering of Natural Product Biosynthetic Pathways,Trends in Biotechnology, 2020, 38(7), 715-728.(IF=19.536,生物工程与应用微生物一区)

4.Song, C.#; Luan, J.#; Cui, Q.; Duan, Q.; Li, Z.; Gao, Y.; Li, R.; Li, A.; Shen, Y.; Li, Y.; Stewart, A. F.; Zhang, Y.*; Fu, J.* &Wang, H.*(主通讯作者), Enhanced heterologous spinosad production from a 79-kb synthetic multi-operon assembly,ACS Synthetic Biology, 2019, 8(1), 137-147.(IF=5.110,生化研究方法二区)

5.Wang, H.(第一作者); Li, Z.; Jia, R.; Yin, J.; Li, A.; Xia, L.; Yin, Y.; Muller, R.; Fu, J.*; Stewart, A. F.* & Zhang, Y.*, ExoCET: exonuclease in vitro assembly combined with RecET recombination for highly efficient direct DNA cloning from complex genomes,Nucleic Acids Research, 2018, 46(5), e28.(IF=16.971,生化与分子生物学一区)

6.Wang, H.#(并列第一作者); Li, Z.#; Jia, R.; Hou, Y.; Yin, J.; Bian, X.; Li, A.; Muller, R.; Stewart, A. F.*; Fu, J.* & Zhang, Y.*, RecET direct cloning and Redab recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression,Nature Protocols, 2016, 11(7), 1175-1190.(IF=13.491,生化研究方法一区)

7.Wang, H.(第一作者); Bian, X.; Xia, L.; Ding, X.; Müller, R.; Zhang, Y.*; Fu, J.* & Stewart, A. F.*, Improved seamless mutagenesis by recombineering usingccdBfor counterselection,Nucleic Acids Research, 2014, 42(5), e37.(IF=16.971,生化与分子生物学一区)

8.Jing, X.#; Cui, Q.#; Li, X.; Yin, J.; Ravichandran, V.; Pan, D.; Fu, J.; Tu, Q.;Wang, H.*(并列通讯作者); Bian, X.* & Zhang, Y.*, EngineeringPseudomonas protegensPf-5 to improve its antifungal activity and nitrogen fixation,Microbial Biotechnology, 2020, 13(1), 118-133.(IF=5.813,生物工程与应用微生物二区)

9.Luan, J.#; Li, Z.#;Wang, H.*(独立通讯作者); Fu, J. & Zhang, Y.,In vitroassay revealed mismatches between guide RNA and target DNA can enhance Cas9 nuclease activity,Current Chinese Science, 2020, 1(1), 69-72.

10.Zhang, Y.#; Liu, R.#; Tian, K.#; Wang, Z.#; Yang, X.; Gao, D.; Zhang, Y.; Fu, J.*;Wang, H.* (并列通讯作者)& Zhao, J.*, Fiber2 and hexon genes are closely associated with the virulence of the emerging and highly pathogenic fowl adenovirus 4,Emerging Microbes & Infections, 2018, 7(1), 199.(IF=7.163,微生物学二区)

11.Wang, H.(第一作者); Yao, T.; Cai, M.; Xiao, X.; Ding, X. & Xia, L.*, A genome walking strategy for the identification of nucleotide sequences adjacent to known regions,Biotechnology Letters, 2013, 35(2), 279-284.

12.Rostovskaya, M.1, Fu, J., Obst, M., Baer, I., Weidlich, S.,Wang, H., Smith, A. J. H., Anastassiadis, K.* & Stewart, A. F.*, Transposon-mediated BAC transgenesis in human ES cells,Nucleic Acids Research, 2012, 40(19), e150.

13.Zhang, W.1, Fu, J.1, Liu, J.,Wang, H., Schiwon, M., Janz, S., Schaffarczyk, L., von der Goltz, L., Ehrke-Schulz, E., Domer, J., Solanki, M., Boehme, P., Bergmann, T., Lieber, A., Lauber, C., Dahl, A., Petzold, A., Zhang, Y.*, Stewart, A. F.* & Ehrhardt, A.*, An engineered virus library as a resource for the spectrum-wide exploration of virus and vector diversity,Cell Reports, 2017, 19(8), 1698-1709.

14.Zhang, W.1, Fu, J., Liu, J.,Wang, H., Schulz, E., Solanki, M., Boehme, P., Gebbing, M., Bergmann, T., Doerner, J., Stewart, A. F. & Ehrhardt, A.*, High-throughput cloning and tagging of an engineered adenovirus-library empowers vector diversity for broad applications,Molecular Therapy, 2015, 23, S21.

15.Bian, X.1, Tang, B.1, Yu, Y., Tu, Q., Gross, F.,Wang, H., Li, A., Fu, J., Shen, Y., Li, Y., Stewart, A. F., Zhao, G., Ding, X.*, Muller, R.* & Zhang, Y.*, Heterologous production and yield improvement of epothilones inBurkholderialesstrain DSM 7029,ACS Chemical Biology, 2017, 12(7), 1805-1812.

16.Yin, J.1,Wang, H., Li, R., Ravichandran, V., Bian, X., Li, A., Tu, Q., Stewart, A.F., Fu, J.* & Zhang, Y.*, A practical guide to recombineering inPhotorhabdusandXenorhabdus,Current Topics in Microbiology and Immunology, 2017.

17.Ravichandran, V.1, Zhong, L.,Wang, H., Yu, G., Zhang, Y.*& Li, A.*, Virtual screening and biomolecular interactions of CviR-based quorum sensing inhibitors against chromobacterium violaceum,Frontiers in Cellular and Infection Microbiology, 2018, 8, 292.

18.Liu, Q.1, Shen, Q.1, Bian, X., Chen, H., Fu, J.,Wang, H., Lei, P., Guo, Z., Chen, W.*, Li, D.* & Zhang, Y.*, Simple and rapid direct cloning and heterologous expression of natural product biosynthetic gene cluster inBacillus subtilisvia Red/ET recombineering,Scientific Reports, 2016, 6, 34623.

19.Jiang, B.1, Zhang, R., Feng, D., Wang, F., Liu, K., Jiang, Y., Niu, K., Yuan, Q., Wang, M.,Wang, H., Zhang, Y. & Fang, X.*, A Tet-on and Cre-loxP based genetic engineering system for convenient recycling of selection markers inPenicillium oxalicum,Frontiers in Microbiology, 2016, 7, 485.

20.Zhu, L.1, Yue, X., Han, K., Li, Z., Zheng, L., Yi, X.,Wang, H., Zhang, Y. & Li, Y.*, Allopatric integrations selectively change host transcriptomes, leading to varied expression efficiencies of exotic genes inMyxococcus xanthus,Microbial Cell Factories, 2015, 14, 105.

21.Bian, X.1, Huang, F.,Wang, H., Klefisch, T., Muller, R.* & Zhang, Y.*, Heterologous production of glidobactins/luminmycins inEscherichia coliNissle containing the glidobactin biosynthetic gene cluster fromBurkholderiaDSM7029,Chembiochem, 2014, 15(15), 2221-2224.

22.Yu, F.1, Jing, X., Li, X.,Wang, H., Chen, H., Zhong, L., Yin, J., Pan, D., Yin, Y., Fu, J., Xia, L., Bian, X., Tu, Q. & Zhang, Y., Recombineering Pseudomonas protegens CHA0: An innovative approach that improves nitrogen fixation with impressive bactericidal potency,Microbiology Research, 2019, 218, 58-65.

23.Fu, J.#; Bian, X.#; Hu, S.;Wang, H.; Huang, F.; Seibert, P. M.; Plaza, A.; Xia, L.; Müller, R.*; Stewart, A. F.* & Zhang, Y.*, Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting,Nature Biotechnology, 2012, 30(5), 440-446.

人才称号

1.国家自然科学基金委优青,2021.8

2.山东省泰山学者(青年专家),2019.1

3.山东大学齐鲁青年学者,2018.7


获奖情况

1.教育部自然科学二等奖,第二位,2019.12.

2.山东大学微生物技术研究院“科研之星”,2021.1

3.山东省大学生生物学大赛二等奖优秀指导教师,2020.10.

4.山东大学青岛校区第三届十佳书院导师,2020.11.

5.山东大学青岛校区第二届十佳书院导师,2019.10.

6.山东大学重大科研突出成绩奖励,2017.12.

7.中国微生物学会学术年会 优秀学术论文交流奖,2014.08.


发明专利

1.基因组大片段直接克隆和DNA多分子组装新技术,中国发明专利,授权号:ZL201710177676.1,张友明;王海龙;符军;Francis Stewart

2.New technique for genomic large fragment direct cloning and DNA multi-molecular assembly,欧洲发明专利,申请号:17901979.9,张友明;王海龙;符军;Francis Stewart

3.Method of homologous recombination of DNA,美国发明专利,申请号:16578385,张友明;王海龙;符军;Francis Stewart

4.王海龙,宋超逸,栾霁,符军,张友明. 一种大型基因簇的无痕定点改造方法及其应用,中国发明专利,申请号: CN202010147911.2

5.王海龙,宋超逸,符军,张友明. 一种含有多杀菌素多操纵子人工基因簇质粒的构建方法与应用,中国发明专利,申请号: CN201811407515.8