医药微生物技术

徐海

教授
博士/硕士生导师
0532-58630658
个人简介 科研项目 学术论文 其他介绍

教育背景

博士 起止时间:2000-2004毕业院校:山东大学 专业:微生物学

硕士 起止时间:1996-1999毕业院校:山东大学 专业:微生物学

学士 起止时间:1987-1992毕业院校:复旦大学 专业:微生物学

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工作经历

起止时间 单位名称 专业技术职务

1992-今 山东大学 教授

2004-2007 英属哥伦比亚大学 博士后

1982-1992 江苏省微生物研究所 助理研究员

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研究方向

1. 抗生素耐药性机制、环境中的传播机制与应对措施

2. 人体微生物组与疾病的关系

科研项目

1.国家自然科学基金国际(地区)合作与交流项目“中英抗菌药物耐药研讨会”,No. 31881230740,2018年10月-2018年12月,项目负责人,1.5万元。

2.国家自然科学基金国际(地区)合作与交流项目“中国-新西兰非传染病双边研讨会”,No. 31781220657,2017年10月-2017年12月,项目负责人,2万元。

3.国家自然科学基金面上项目“大肠杆菌cAMP-CRP途径调控1型整合子中抗生素耐药基因表达的机制研究”,No.31770042,2018年1月-2021年12月,项目负责人,60万元。

4.国家重大科技专项子课题“超耐药菌在食品生产链中的流行病学及防控技术研究”,No.2013ZX10004217003, 2013年1月-2015年12月,项目负责人, 17万元。

5.国家自然科学基金面上项目“质粒介导的喹诺酮类耐药基因在环境污水中传播及相关耐药肠道细菌的遗传结构分析”,No.31170112, 2012年1月-2015年12月,项目负责人,55万元。

6.山东省医药卫生科技发展计划项目“从环境污水中分离、鉴定细菌质粒上与整合子相关的耐药基因”,No.2011HZ050, 2011年9月-2013年1月,项目负责人,1万元。

7.国家自然科学基金面上项目“水环境中分离耐抗生素细菌及整合子结构的遗传分析”,No.30870084, 2009年1月-2013年1月,项目负责人,30万元。

8.国家自然科学基金重点项目“组学工具解析斜卧青霉纤维素酶系合成调控网络机制研究”,No.31030001, 2010年1月-2013年12月,参与,212万元。

9.国家自然科学基金面上项目“问号钩端螺旋体异亮氨酸合成新途径的分子遗传机理”,No.30470018, 2005年1月-2007年12月,项目负责人,26万元。

代表性论文

1. Li L, Yu T, Ma Y, Yang Z, Wang W, Song X, Shen Y, Guo T, Kong J, Wang M *,Xu H *.2019. The genetic structures of an Extensively Drug Resistant (XDR)Klebsiella pneumoniaeand its plasmids. Front Cell Infect Microbiol 8: 446.

2. Wang M1, Ma Y1, Li L, Wang B, Wei X, Zhang M, Wang J, Cui Q, Li Z *,Xu H *. 2019. The diversity of glycosylation of cellobiohydrolase I fromTrichoderma reeseidetermined with mass spectrometry. Biochem Biophys Res Commun 508(3): 818-824.

3. Bie L, Fang M, Li Z, Wang M *,Xu H*. 2018. Identification and characterization of new resistance-conferring SGI1s (Salmonella genomic island 1) inProteus mirabilis. Front Microbiol 9: 3172.

4. Wan X, Gao M, Wang Y, Xu H, Wang M, Wang X*. 2017. Formation, characteristics and microbial community of aerobic granular sludge in the presence of sulfadiazine at environmentally relevant concentrations. Bioresour Technol250:486-494.

5. Yuan S, Gao M, Zhu F, Afzal MZ, Wang Y,Xu H, Wang M, Wang S, Wang X*. 2017. Disintegration of aerobic granules during prolonged operation. Environ Sci: Water Res Technol 3:757.

6.Wang M1, Shen W1, Yan L, Wang X, Xu H*. 2017. Stepwise impact of urban wastewater treatment on bacterial community structure, content of antibiotics, and prevalence of antimicrobial resistance. Environ Pollut 231(P2):1578-1585.

7.李紫云,王明钰,徐海*.细菌II型、III型整合子在耐药性传播中的作用[J].中国抗生素杂志, 2018,43(2):156-162.

8.崔庆宇,王明钰,徐海*.lux荧光报告系统的研究进展[J].中国生物工程杂志, 2017,37(8):66-71.

9. Guo N, Wang Y*, Yan L, Wang X, Wang M,Xu H, Wang S*. 2017. Effect of bio-electrochemical system on the fate and proliferation of antibiotic resistance genes during the treatment of chloramphenicol wastewater. Water Res117:95-101.

10.马亚楠,王明钰*,徐海.纤维素酶糖基化的研究进展[J].微生物学报, 2017,57(8):1-8.

11.Gao M, Diao M, Yuan S, Wang Y,Xu H, Wang X*. 2017. Effects of phenol on physicochemical properties and treatment performances of partial nitrifying granules in sequencing batch reactors. Biotechnol Rep13:13-18.

12.Bie L, Wu H, Wang X, Wang M*,Xu H*. 2017. Identification and Characterization of new SXT/R391 family integrative and conjugative elements (ICEs) inProteus mirabilisisolates from broiler carcasses. Int J Antimicrob Agents50(2):242-246.

13.Yan L, Liu D, Wang X, Wang Y, Wang M*,Xu H*. 2017. Bacterial plasmid-mediated quinolone resistance genes in aquatic environments in China. Sci Rep7:40610.

14.Wu H, Wang M,Liu Y, Wang X, Wang Y, Lu J,Xu H*. 2016. Data on the phylogenetic typing, integron gene cassette array analysis, multi-drug resistance analysis and correlation between antimicrobial resistance determinants inKlebsiellastrains. Data Brief8:1289-1294.

15.闫雷,徐海*.质粒介导的喹诺酮耐药基因qnr的分类、耐药机制及其在国内的流行状况[J].微生物学报, 2016, 56(2):169-179.

16.沈伟涛,王明钰,王允坤,王新华,徐海*.抗生素含量测定方法的分析综述[J].中国生物工程杂志, 2016, 36(6):119-126.

17.Wu H, Wang M, Liu Y, Wang X, Wang Y, Lu J,Xu H*. 2016. Characterization of antimicrobial resistance inKlebsiellaspecies isolated from chicken broilers. Int J Food Microbiol232:95-102.

18.王娟,王新华,徐海*.多重耐药菌在人类、动物和环境的耐药和传播机制[J].微生物学报, 2016, 56(11):1671-1679.

19.Wu H1, Xia S1, Bu F, Qi J, Liu Y,Xu H*. 2015. Identification of integrons and phylogenetic groups of drug-resistantEscherichia colifrom broiler carcasses in China. Int J Food Microbiol211:51-56.

20.别路垚,徐海*.细菌中整合性结合元件的研究进展[J].微生物学通报, 2015, 42(11):2215-2222.

21.欧阳润泽,贺艳艳,徐海*.细菌整合子中整合酶基因调控的研究进展[J].微生物学报, 2015, 55(10):1231-1237.

22.Liu G1, Zhang L1, Qin Y1, Zou G1, Li Z1, Yan X, Wei X, Chen M, Chen L, Zheng K, Zhang J, Ma L, Li J, Liu R,Xu H, Bao X, Fang X, Wang L, Zhong Y, Liu W, Zheng H, Wang S, Wang C, Xun L, Zhao G*, Wang T*, Zhou Z*, Qu Y*. 2013. Long-term strain improvements accumulate mutations in regulatory elements responsible for hyper-production of cellulolytic enzymes. Sci Rep.3:1569.

23.Xia R1, Ren Y1,Xu H*. 2013. Identification of plasmid-mediated quinolone

resistanceqnrgenes in multidrug-resistant Gram-negative bacteria from hospital wastewaters and receiving waters in the Jinan area, China. Microb Drug Resist19(6):446-456.

24.Xia R, Ren Y, Guo X,Xu H*. 2013. Molecular diversity of class 2 integrons in antibiotic-resistant gram-negative bacteria found in wastewater environments in China. Ecotoxicology22:402-414.

25.Allen KJ*, Laing CR, Cancarevic A, Zhang Y, Mesak LR,Xu H, Paccagnella A, Gannon VPJ, Hoang L. 2013. Characteristics of clinical shiga toxin-producingEscherichia coliisolated from British Columbia. Biomed Res Int2013:878956.

26.Han N, Sheng D,Xu H*. 2012. Role ofEscherichia colistrain subgroups, integrons, and integron-associated gene cassettes in dissemination of antimicrobial resistance in aquatic environments of Jinan, China. Water Sci Technol66(11):2385-2392.

27.Guo X1, Xia R1, Han N,Xu H*. 2011. Genetic diversity analyses of class 1 integrons and their associated antimicrobial resistance genes inEnterobacteriaceaestrains recovered from aquatic habitats in China. Lett Appl Microbiol52:667-675.

28. Xu H*, Miao V, Kwong W, Xia R, Davies J. 2011. Identification of a novel fosfomycin resistance gene (fosA2) inEnterobacter cloacaefrom the Salmon River, Canada. Lett Appl Microbiol52:427-429.

29. Xu H*, Broersma K, Miao V, Davies J. 2011. Class 1 and class 2 integrons in multidrugresistant gram-negative bacteria isolated from the Salmon River, British Columbia. Can J Microbiol57:460-467.

30.Xia R, Guo X, Zhang Y,Xu H*. 2010.qnrVC-like gene located in a novel complex Class 1 integron harboring the ISCR1element in anAeromonas punctatastrain from an aquatic environment in Shandong province, China. Antimicrob Agents Chemother54(8):3471-3474.

31.国宪虎,夏蕊蕊,徐海*.整合子基因盒系统及β-内酰胺酶介导的细菌耐药[J].微生物学通报, 2010, 37(2):289-294.

32.夏蕊蕊,国宪虎,张玉臻,徐海*.喹诺酮类药物及细菌对其耐药性机制研究进展[J].中国抗生素杂志, 2010, 35(4):255-261.

33.Zhang P1, Ma J1, Zhang Z, Zha M,Xu H, Zhao G*, Ding J*. 2009. Molecular basis of the inhibitor selectivity and insights into the feedback inhibition mechanism of citramalate synthase fromLeptospira interrogans. Biochem J421:133-143.

34.Ma J1, Zhang P1, Zhang Z, Zha M,Xu H, Zhao G*, Ding J*. 2008. Molecular basis of the substrate specificity and the catalytic mechanism of citramalate synthase fromLeptospira interrogans. Biochem J415:45-56.

35. Xu H, Davies J, Miao V*. 2007. Molecular characterization of Class 3 integrons fromDelftiaspp. J Bacteriol189(17):6276-6283.

36.Zou Y, Guo X, Picardeau M,Xu H, Zhao G*. 2007. Acomprehensive survey on isoleucine biosynthesis pathways in seven epidemicLeptospira interrogansreference strains of China. FEMS Microbiol Lett269:90-96.

37. Xu H, Zhang Y, Guo X, Ren S, Staempfli AA, Chiao J, Jiang W, Zhao G*. 2004. Isoleucine biosynthesis inLeptospira interrogansSerotype lai strain 56601 proceeds via a threonine-independent pathway. J Bacteriol186(16):5400-5409.

38.Ren S1, Fu G1, Jiang X1, Zeng R1, Miao Y,Xu H, Zhang Y, Xiong H, Lu G, Lu L, Jiang H, Jia J, Tu Y, Jiang J, Gu W, Zhang Y, Cai Z, Shen H, Yin H, Zhang Y, Zhu G, Wan M, Huang H, Qian Z, Wang S, Ma W, Yao Z, Shen Y, Qiang B, Xia Q, Guo X, Danchin A, Girons IS, Somerville RL, Wen Y, Shi M, Chen Z, Xu J, Zhao G*. 2003. Unique physiological and pathogenic features ofLeptospira interrogansrevealed by whole-genome sequencing. Nature422:888-893.

39.徐海,钱卫,季明杰,巩海涛.核苷酸补救途径在肌苷代谢中应用的研究[J].山东大学学报(自然科学版), 2000, 35(4):453-457.

40.徐海,钱卫,朱明田,蔡春萍,高培基.酸水解麸皮对斜卧青霉产纤维素酶的影响[J].食品与发酵工程, 1997, 23(1):15-18.

41.徐海,钱卫,高东,周旭宇.红酵母产生类胡萝卜素的研究[J].工业微生物, 1997, 27(3):24-26.

42.徐海,钱卫,徐慧.肌苷产生菌中降低核苷水解酶的研究[J].生物技术, 1996, 6(5):28-30.

43.. Li Z, Shi L, Wang B, Wei X, Zhang J, Guo T, Kong J,Wang M *, Xu H *. 2020. In vitro assessment of antimicrobial resistance dissemination dynamics during multidrug resistant bacteria invasion events using a continuous culture device. Appl Environ Microbiol doi: 10.1128/AEM.02659-20.

44. Wang M 1, Wang W 1, Niu Y, Liu T, Li L, Zhang M, Li Z, Su W, Liu F, Zhang X *, Xu H *. 2020. A clinical extensively-drug resistant (XDR) Escherichia coli and role of its β-lactamase genes. Front Microbiol 11:590357. doi:10.3389/fmib.2020.590357.

45. Yang Z 1, Cui Q1, Zhang M, Li Z, Wang M*, Hai X*. 2020. A lux-based Staphylococcus aureus bioluminescence screening assay for the detection/identification of antibiotics and prediction of antibiotic mechanisms. J Antibiot 73:828-836. doi: 10.1038/s41429-020-0349-7.

46. Yang Z, Cui Q, An R, Wang J, Song X, Shen Y, Wang M *, Xu H *. 2020. Comparison of microbiomes in ulcerative and normal mucosa of recurrent aphthous stomatitis (RAS)-affected patients. BMC Oral health 20:128. doi: 10.1186/s12903-020-01115-5.

47.Li L, Zhang M, Wang W, Xia R, Ma Y, Wei X, Wang X, Sun X, Xie X, Xie S, Wang M*, Xu H*. 2021. Identification and characterization of two novel ISCR1-associated genes dfrA42 and dfrA43 encoding trimethoprim resistant dihydrofolate reductases. Antimicrob Agents Chemother 65(6):e02010-20.

获奖情况

1. 纤维素酶液体深层发酵,国家发明四等奖,1998年

2.纤维素酶液体深层发酵,国家教委科技进步一等奖,1997年

3.肌苷发酵技术,山东省高校发明优秀奖,1995年


所获专利

1.徐海,国宪虎,夏蕊蕊.利用EcoRII酶切图谱库检测细菌整合子中基因盒阵列的方法:中国,201110080541.6[P].

2.朱明田,李波,徐海,钱卫,曲音波, 高培基.高活性纤维素酶的制造方法:中国,96116049.7[P].