医药微生物技术

李越中

教授
博士/硕士生导师
0532-58631539
个人简介 学术论文 其他介绍

教育背景

博士 起止时间:1990-1993,毕业院校:山东大学,专业:微生物学

硕士 起止时间:1987-1990,毕业院校:山东农业大学,专业:真菌学

学士 起止时间:1983-1987,毕业院校:山东大学,专业:微生物学


工作经历

起止时间 单位名称 专业技术职务

1993-1995 中科院沈阳应用生态所 博士后

1995-2000 山东大学微生物技术国家重点实验室 副教授

1996.4-1996.10 德国国家生物技术研究中心(GBF) 高级访问学者

2000-今 山东大学微生物技术国家重点实验室 教授


研究方向

黏细菌(Myxobacteria)在系统进化上属于Proteobacteria类群的δ分支。在其生活史中表现复杂的多细胞群体行为特性,显示与多细胞真核生物较多的相似性,显著不同于其他细菌,被认为是生活于多细胞生命边缘的单细胞生物类群,是一类“高等”的原核生物,是细菌细胞间通讯与协作,多细胞形态发生,以及生命进化等研究的重要模式生物材料。黏细菌能够降解利用其他活的微生物细胞、细胞残体以及许多难降解的大分子有机物,是一类捕食性细菌,在微生物生态系统扮演重要角色。黏细菌拥有庞大的基因组,是目前报道的原核生物中最大的。黏细菌能够产生丰富的次级代谢产物,具有种类多、结构新、作用机理新颖多样的特性,被公认是继放线菌之后又一个重要的药源微生物新类群。

我们以黏细菌为基本材料,开展如下方面的研究:

1.黏细菌资源及其多样性,包括可培养和未培养的黏细菌类群;

2.以黏细菌资源为基础,开展黏细菌细胞行为及其进化研究;

3.以黏细菌产生的抗肿瘤化合物埃博霉素(Epothilones)为主要研究对象,开展黏细菌发酵工程和代谢工程研究;

4.在黏细菌中建立和改进遗传操作体系,并对黏细菌进行遗传分析和遗传改造。

代表性论文(近五年)

Yuelan Li#, Li Zhuo#, Xiaobin Li, Yongqiang Zhu, Shuge Wu, Wei Hu, Tao Shen, Yue-Zhong Li*, changsheng Wu*. Myxadazoles, myxobacterium-derived isoxazole-benzimidazole hybrids with cardiovascular activities.Angewandte Chemie International Edition2021, doi.org/10.1002/anie.202106275.

Jingjing Wang#, Jianing Wang#, Shuge Wu, Zheng Zhang*, Yuezhong Li*. Global geographic diversity and distribution of the myxobacteria.Microbiology Spectrum2021, 9: e00012-21.

Jianing Wang, Lidong Wang, Weifeng Hu, Zhuo Pan, Peng Zhang, Chuandong Wang, Jingjing Wang, Shuge Wu, Yue-zhong Li*. Assembly processes and source tracking of planktonic and benthic bacterial communities in the Yellow River estuary.Environmental Microbiology2021, 23(5), 2578–2591.

Jia-Qi Hu#, Jing-Jing Wang#, Yue-Lan Li, Li Zhuo, Ai Zhang, Hai-Yan Sui, Xiao-Ju Li, Tao Shen, Yizhen Yin, Zhi-Hong Wu, Wei Hu, Yue-Zhong Li*, Changsheng Wu*. Combine NMR-basedmetabolic profiling andgenome mining for the accelerated discovery of archangiumide, an allenic macrolide from the myxobacteriumArchangium violaceum SDU8.Organic Letters2021, 23, 2114?2119.

Ya Liu, Jianing Wang, Zheng Zhang, Feng Wang, Ya Gong, Duo-hong Sheng, Yue-zhong Li*. Two PAAR proteins with different C-terminal extended domains have distinct ecological functions in Myxococcus xanthus.Applied and Environmental Microbiology2021, 87: e00080-21.

Duohong Sheng*, Ye Wang, Zhiwei Jiang, Dongkai Liu, Yuezhong Li*. ImuA facilitates SOS mutagenesis by inhibiting RecA-mediated activity in Myxococcus xanthus.Applied and Environmental Microbiology2021, doi:10.1128/AEM.00919-21.

Zhuo Pan, Zheng Zhang, Li Zhuo, Tian-yu Wan, Yue-zhong Li*. Bioinformatic and functional characterization of Hsp70s in Myxococcus xanthus.mSphere2021, 6: e00305-21.

DH Sheng,Y Wang,SG Wu,RX Duan,YZ Li*. The regulation of LexA on UV induced SOS response in Myxococcus xanthus based on transcriptome analysis.Journal of Microbiology and Biotechnology, 2021, 31(6).

Zheng Zhang#*, Jianing Wang#, Jinlan Wang, Jingjing Wang, Yuezhong Li*. Estimate of the sequenced proportion of the global prokaryotic genome.Microbiome. 2020 Sep 16;8(1):134.

Wang, J. N., Wang, J. J., Zhang, Z., Li, Z., Li, Y. Z*. Shifts in the Bacterial Population and Ecosystem Functions in Response to Vegetation in the Yellow River Delta Wetlands.mSystems, 2020, 5(3): e00412-20

Y Liu, Z Zhang, F Wang, DD Li, YZ Li*. Identification of type VI secretion system toxic effectors using adaptors as markers.Computational and Structural Biotechnology Journal, 2020, 18(6):3723-3733.

DH Sheng, YX Wang, M Qiu, JY Zhao, YZ Li*. Functional Division Between the RecA1 and RecA2 Proteins in Myxococcus xanthus.Frontiers in Microbiology, 2020, 11:140.

XJ Chen, Z Zhang, YJ Li, L Zhuo, DH Sheng, YZ Li*. Insights into the persistence and phenotypic effects of the endogenous and cryptic plasmid pMF1 in its host strain Myxococcus fulvus 124B02.FEMS Microbiology Ecology, 2020(3):3.

Peng Zhang, Lijuan Zhang, Xin-jing Yue, Ya-jie Tang, Changsheng Wu*, Yue-zhong Li*. Effects of glycosylation on the bioactivity of rapamycin.Applied Microbiology and Biotechnology, 2020, 104(21):9125–9134

SG Wu, JJ Wang, JN Wang, Q Chen, YZ Li*. Psychroflexus aurantiacus sp. nov. isolated from soil in the Yellow River Delta wetlands.International Journal of Systematic and Evolutionary Microbiology, 2020, 70(12).

SG Wu, JJ Wang, JN Wang, Q Chen, YZ Li*. Paucihalobacter ruber gen. nov. sp. nov. isolated from a haloalkaline lake sediment sample.International Journal of Systematic and Evolutionary Microbiology, 2020, 70(10).

JJ Wang, SG Wu, Q Chen, DH Sheng, YZ Li*. Deinococcus terrestris sp. nov. a gamma ray- and ultraviolet-resistant bacterium isolated from soil.International Journal of Systematic and Evolutionary Microbiology, 2020, 70(9).

Peng Zhang, Zheng Zhang, Zhi-feng Li, Qi Chen, Yao-yao Li, Ya Gong, Xin-jing Yue, Duo-hong Sheng, You-ming Zhang, Changsheng Wu*, Yue-zhong Li*. Phylogeny-guided characterization of glycosyltransferases for epothilone glycosylation.Microbial Biotechnology, 2019, 12(4), 763-774

Mian Nabeel Anwar, Zhi Feng Li, Gong Ya, Raghvendra Partap Singh, Yue-zhong Li*. Omics studies revealed the factors involved in the formation of colony boundary in Myxococcus xanthus.Cells, 2019, 8, 530.

Li Zhuo, Zheng Zhang, Zhuo Pan, Duo-hong Sheng, Wei Hu, Yue-zhong Li*. CIRCE element evolved for the coordinated transcriptional regulation of bacterial duplicate groELs.Biochimica et Biophysica Acta - Gene Regulatory Mechanisms. 2018 Oct;1861(10):928-937.

Ya Gong, Zheng Zhang, Ya Liu, Xiu-wen Zhou, Mian Nabeel Anwar, Ze-shuo Li, Wei Hu, Yue-zhong Li*. A nuclease-effector and immunity system for the kin discrimination in Myxococcus xanthus.Environmental Microbiology. 2018, doi: 10.1111/1462-2920.14282.

Ya Gong, Zheng Zhang, Xiu-wen Zhou, Mian Nabeel Anwar, Xiao-zhuang Hu, Ze-shuo Li, Xiao-jing Chen, Yue-zhong Li*. Competitive interactions between incompatible mutants of the social bacterium Myxococcus xanthus DK1622.Frontiers in Microbiology. 2018, 9:1200.

Zheng Zhang, Jin-Lan Wang, Ya Gong, Yue-Zhong Li*. Contributions of substitutions and indels to the structural variations in ancient protein superfamilies.BMC Genomics. 2018 Oct 24;19(1):771.

Li Y, Liu Y, Zhang Z, Chen X, Gong Y and Li Y*. A Post-segregational Killing Mechanism for Maintaining Plasmid PMF1 in Its Myxococcus fulvus Host.Frontiers in Cellular and Infection Microbiology. 2018 Aug 7; 8:274.

JJ Wang, Q Chen, YZ Li*. Chryseolinea flava sp. nov., a new species of Chryseolinea isolated from soil.International Journal of Systematic and Evolutionary Microbiology. 2018 Nov;68(11):3518-3522.

XJ Yue, XW Cui, Z Zhang, WF Hu, ZF Li, YM Zhang and YZ Li*. Effects of transcriptional mode on promoter substitution and tandem engineering for the production of epothilones in Myxococcus xanthus.Applied Microbiology Biotechnology. (2018) doi.org/10.1007/s00253-018-9023-4

R Peng, Y Wang, WW Feng, XJ Yue, JH Chen, XZ Hu, ZF Li, DH Sheng, YM Zhang and YZ Li*. CRISPR/dCas9?mediated transcriptional improvement of the biosynthetic gene cluster for the epothilone production in Myxococcus xanthus.Microbial Cell Factories(2018) 17:15.

L Zhuo, Y Wang, Z Zhang, J Li, XH Zhang, YZ Li*. Myxococcus xanthus DK1622 coordinates expressions of the duplicate groEL and single groES genes for synergistic functions of GroELs and GroES.Frontiers in Microbiology(2017) 8: 733.

R Peng, JH Chen, WW Feng, Z Zhang, J Yin, ZS Li, YZ Li*. Error-prone DnaE2 balances the genome mutation rates in Myxococcus xanthus DK1622.Frontiers in Microbiology(2017) 8: 122.

XJ Yue, XW Cui, Z Zhang, R Peng, P Zhang, ZF Li, YZ Li*. A bacterial negative transcription regulator binding on an inverted repeat in the promoter for epothilone biosynthesis.Microbial Cell Factories(2017) 16: 92.

YJ Yang, Y Wang, ZF Li, Y Gong, P Zhang, WC Hu, DH Sheng and YZ Li*. Increasing on target cleavage efficiency for CRISPR/Cas9 induced large fragment deletion in Myxococcus Xanthus.Microbial Cell Factories(2017) 16:142.


获奖情况

山东省科技发明二等奖(2006)

国家杰出青年科学基金资助(2008)

“百千万”工程国家级人才(2009)

教育部高等学校自然科学一等奖(2017)

山东省首批领军人才创新工作室(2017)