医药微生物技术

卞小莹

教授
博士/硕士生导师
0532-58632596
个人简介 科研项目 学术论文 其他介绍

 

 



教育背景

博士 2008.10-2012.10 德国萨尔大学 药物生物技术

硕士 2005.09-2008.07 西北农林科技大学 天然产物农药

学士 2001.09-2005.07 西北农林科技大学 植物保护


工作经历

起止时间 单位名称 专业技术职务

2015.07-至今 山东大学微生物技术国家重点实验室 教授

2014.10-2015.06 山东大学微生物技术国家重点实验室 专职研究人员

2012.10-2014.09 德国亥姆霍兹萨尔药物研究所 博士后

 


研究方向

1.微生物基因组编辑技术

2.微生物天然产物挖掘与改造

3. 微生物天然产物组合生物合成及合成生物学


科研项目

2021/07-2024/06高效微生物细胞工厂的设计原理与构建方法,国家重点研发计划,2021YFC2100500,骨干

2021/01-2024/12伯克氏菌中新型非核糖体脂肽的定向挖掘与途径解析,国家自然科学基金面上项目,32070060,主持

2020/01-2024/12天然产物“暗物质”定向挖掘的平台技术, 国家重点研发计划课题,2019YFA0905701,主持

2019/07-2022/06微生物次级代谢,山东省自然科学杰出青年基金,ZR2019JQ11,主持

2017/09-2019/08, 兼具生物固氮/杀虫抑菌功能的根际细胞工厂的构建, 山东省自然科学基金重大基础研究项目,ZR2017ZB0212,主持

2017/01-2020/12,一个用于表达黏细菌天然产物的底盘菌的优化及应用,国家自然科学基金面上项目,31670098,主持

2016/01-2018/12,创新霉素生物合成途径的阐释和异源表达,国家自然科学基金青年项目,31500033,主持

2015/7-2020/7,山东大学齐鲁青年学者学科建设经费,主持


代表性论文

Liu J, Zhou H, Yang Z, Wang X, Chen H, Zhong L, Zheng W, Niu W, Wang S, Ren X, Zhong G, Wang Y, Ding X, Müller R, Zhang Y,Bian X*. Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria.Nat Commun. 2021,12: 4347. DOI : 10.1038/s41467-021-24645-0.

Zhong L#,DiaoX#,Zhang N#, Li F, Zhou H, Chen H, Bai X, Ren X, Zhang Y*, Wu D*,Bian X*.Engineering and Elucidation of the Lipoinitiation Process in Nonribosomal Peptide Biosynthesis.Nat Commun.2021, 12: 296.被Nat Commun编辑选为高亮文章.

Chen H, Sun T, Bai X, Yang J, Yan F, Yu L, Tu Q, Li A, Tang Y, Zhang Y*,Bian X*, Zhou H*. Genomics-Driven Activation of Silent Biosynthetic Gene Clusters inBurkholderia gladioliby Screening Recombineering System.Molecules. 2021,26(3): 700.

Ouyang Q, Wang X, Zhang N, Zhong L, Liu J, Ding X, Zhang Y,Bian X*. Promoter screening facilitates heterologous production of complex secondary metabolites in Burkholderiales strains.ACS Synth Biol.2020, 9(2):457-460.

Wang ZJ, Zhou H, Zhong G, Huo L, Tang YJ, Zhang Y*,Bian X*. Genome Mining and Biosynthesis of Primary Amine-Acylated Desferrioxamines in a Marine Gliding Bacterium.Org Lett. 2020, 22(3): 939-943.

Zhong, L.; Ravichandran, V.; Zhang, N.; Wang, H.;Bian, X*; Zhang, Y*; Li, A*. Attenuation of Pseudomonas aeruginosa Quorum Sensing by Natural Products: Virtual Screening, Evaluation and Biomolecular Interactions.Int J Mol Sci2020, 21, 2190.

Chen H#, Zhou H#, Sun T, Xu J, Tu Q, Yang J, Zhang Y,Bian X*. Identification of holrhizins E-Q reveals the diversity of nonribosomal lipopeptides in Paraburkholderia rhizoxinica.J Nat Prod.2020, 83(2):537-541.

Zheng W#, Wang X#, Zhou H, Zhang Y*, Li A*,Bian X*. Establishment of recombineering genome editing system in Paraburkholderia megapolitana empowers activation of silent biosynthetic gene clusters.Microb Biotechnol.2020, 13(2): 397-405.被Microb Biotechnol(2020, 13:1702-1704)的Highlight栏目重点介绍。

Jing X#, Cui Q#, Li X, Yin J, Ravichandran V, Pan D, Fu J, Tu Q, Wang H*,Bian X*, Zhang Y*. Engineering Pseudomonas protegens Pf-5 to improve its antifungal activity and nitrogen fixation.Microb Biotechnol. 2020, 13(1): 118-133.

Huo L, Hug JJ, Fu C,Bian X*, Zhang Y*, Müller R*. Heterologous expression of bacterial natural product biosynthetic pathways.Nat Prod Rep.2019, 36(10):1412-1436.入选ESI热点论文和高被引论文

Wang X#, Zhou H#, Chen H#, Jing X, Zheng W, Li R, Sun T, Liu J, Fu J, Huo L, Li YZ, Shen Y, Ding X, Müller R,Bian X*, Zhang Y*. Discovery of recombinases enables genome mining of cryptic biosynthetic gene clusters in Burkholderiales species.Proc Natl Acad Sci U S A. 2018, 115 (18): E4255-E4263.被Curr Opin Microbiol (2019, 51: 9-15)和Curr OpinChemBiol(2021, 60: 47-54)推荐。

Xu X, Zhou H, Liu Y, Liu X, Fu J, Li A, Li YZ, Shen Y,Bian X*, Zhang Y*. Heterologous Expression Guides Identification of the Biosynthetic Gene Cluster of Chuangxinmycin, an Indole Alkaloid Antibiotic.J Nat Prod. 2018, 81(4): 1060-1064.

Chen X, Fang Q, Tu Q, Liu C, Yin J, Yin Y, Xia L,Bian X*, Zhang Y*. Identification of a contact-dependent growth inhibition system in the probiotic Escherichia coli Nissle 1917.FEMS Microbiol Lett.2018; 365(11): fny102.

郑文韬,张友明*,卞小莹*。Red/ET同源重组技术及在微生物基因组挖掘中的应用进展。微生物学报, 2017, 57(11): 1735-1746

Bian X#, Tang B#, Yu Y, Tu Q, Gross F, Wang H, Li A, Fu J, Shen Y, Li YZ, Stewart AF, Zhao G, Ding X*, Müller R*, Zhang Y*.Heterologous production and yields improvement of epothilones in the Burkholderiales strain DSM 7029.ACS Chem Biol.2017; 12 (7): 1805-1812. Highlight inHot off the Press of Natural Product Reports(Nat. Prod. Rep., 2017, 34, 1180-1184).

Bian X, Plaza A, Zhang Y*, Müller R*. Two more pieces of the colibactin genotoxin puzzle from Escherichia coli show incorporation of an unusual 1-aminocyclopropanecarboxylic acid moiety.Chem Sci.2015, 6(5): 3154-3160,

Bian X, Plaza A, Yan F, Zhang Y*, Müller R*.Rational and efficient site-directed mutagenesis of adenylation domain alters relative yields of luminmide derivatives in vivo.Biotechnol Bioeng.2015 Jul;112(7):1343-1353.

Bian X, Huang F, Wang H, Klefisch T, Müller R*, Zhang Y*.Heterologous production of glidobactins/luminmycins in Escherichia coli Nissle containing the glidobactin biosynthetic gene cluster from Burkholderia DSM7029.ChemBioChem. 2014, 15(15): 2221-2224.

Ongley SE#,Bian X#, Neilan BA*, Müller R*. Recent advances in the heterologous expression of microbial natural product biosynthetic pathways.Nat Prod Rep. 2013, 30(8):1121-1138.

Bian X, Fu J, Plaza A, Herrmann J, Pistorius D, Stewart AF, Zhang Y*, Müller R*.In vivo evidence for a prodrug activation mechanism during colibactin maturation.ChemBioChem. 2013, 14(10):1194-1197.

Bian X, Plaza A, Zhang Y*, Müller R*. Luminmycins A-C, cryptic natural products from Photorhabdus luminescens identified by heterologous expression in Escherichia coli.J Nat Prod. 2012, 75(9):1652-1655.2012 Arthur E. Schwarting Award for Best Paper in the Journal of Natural Products

Bian X#, Huang F#, Stewart FA, Xia L, Zhang Y*, Müller R*.Direct cloning, genetic engineering, and heterologous expression of the syringolin biosynthetic gene cluster in E. coli through Red/ET recombineering.ChemBioChem.2012, 13(13):1946-1952.

Fu J#,Bian X#, Hu S, Wang H, Huang F, Seibert PM, Plaza A, Xia L, Müller R*, Stewart AF*, Zhang Y*. Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting.Nat Biotechnol. 2012, 30(5): 440-446. Highlighted in‘Cover Paper’ and ‘News and Views’ of Nature Biotechnology,and Research Highlighted inNature Methods


获奖情况

2021年第五届微生物技术国家重点实验室青年人才学术交流会 优秀报告一等奖

2020年第四届微生物技术国家重点实验室青年人才学术交流会 优秀报告二等奖

2019年度教育部自然科学二等奖,Red/ET基因组编辑与微生物资源发掘,排名第三

2019年第三届微生物技术国家重点实验室青年人才学术交流会 优秀报告二等奖

2016年度微生物技术国家重点实验室青年人才学术交流会优秀报告二等奖

2015年中国微生物学会学术年会优秀学术论文交流奖(中国微生物学会)

2013 DECHMA travel grant for 1st European Conference on Natural Products (德国化学工程与生物技术协会)

2012 Arthur E. Schwarting Award for Best Paper in the Journal of Natural Products (美国生药学会)


所获专利

卞小莹,王雪,陈汉娜,周海波,张友明。一组伯克氏菌同源重组酶及其表达载体与应用, ZL201710685563.2

卞小莹,张友明,许晓坤。创新霉素生物合成基因簇及其应用,ZL201710146841.7

Y. Zhang, J. Fu,X. Bian, F. Stewart, R. Müller.Heterologous hosts.US 9,580,717 B2